Hi there! My question is not necessarily specific to R, bit I still hope that I can find help here. I appreciate any suggestions; how to do/improve my analysis, what stuff to read more on etc... I am interested in a dependent variable A. I measured A of 10 individuals at 7 different levels of an explanatory variable B. The levels of B represent a magnitude of force, so in theory, are continuous. I however controlled the exact magnitude of the force and therefore assume it to have 7 discrete levels. The numerical difference between the levels is not equal either; the values are 0, 0.5, 1, 2, 4, 6 and 8. I also measured another continuous variable C of each of the 10 individuals at each of the 7 different levels of B. I am now interested in the following questions: a) To what extend does B influence A? b) To what extend does B influence C? c) To what extend does C influence A? My main problem is that I have several ideas of how to do this analysis, but not the sufficient knowledge to decide which is most appropriate. In a very first step, I simply did a linear regression with B~A and then looked at the residuals of that regression plotted against C. I would guess however, that there is a better way of doing a similar analysis that also allows to account for my repeated measures design and to remove the influence of B on C - maybe an ANCOVA, setting B as a factor and C as a covariate: A~as.factor(B)*C As far as I understand it, I have to account for my repeated measures design, thus include an error term: A~as.factor(B)*C+Error(Individual/as.factor(B)) Now this cannot be quite it, as my repeated measures is also affecting C. C is a continuous variable measured from each of the 10 individuals at each of the 7 levels of B. I am however entirely unsure about how to phrase the error term correctly. Would the corresponding syntax be A~as.factor(B)*C+Error(Individual/C/as.factor(B)) or A~as.factor(B)*C+Error(Individual/(C*as.factor(B))) or maybe something completely different? Then again, maybe that is the wrong way of modelling my data in the first place. I reckon one could argue, that I should model both A and C as dependent variables and do a MANOVA: y <- cbind(A, C) fit <- manova(y~B) Here, however, I don't account for repeated measures and don't know how to extract information about the influence of C on A. Others might argue, that I have to do a mixed effect ANOVA, modelling C as random factor. Or maybe that is wrong, too, and I should in fact do a multiple linear regression, and don't model /B/ as a factor - but how to account for repeated measures here? I read several replies to similar questions, but now am a bit confused about all the stuff I find people recommending in similar situations in the web. What is the correct and best way to do the analysis in my situation? I would really appreciate your help. Best, David [[alternative HTML version deleted]]