Anna Radtke
2010-Oct-29 16:05 UTC
[R] Tukey post hoc comparison (glht?) after 3factorial mixed model (lmer)
Hello, dear R-community. This is a question about TukeyHSD between factor combinations of a Three-Way ANOVA, which is - since it is a multi measure ANOVA - not a simple ANOVA but a Generalised Linear Mixed Model (GLMM), calculated with "lmer".> growth <-groupedData(length~meas|box_id,outer=~spec*comp*water,data=all.spec)> model <- lmer(length~spec*comp*water+(meas|box_id),data=growth) > summary(model)This works fine. But now, I would like to calculate Tukey HSD among certain factor combinations. Formerly (before version 1.0), Tukey contrasts including all levels of interactions could be calculated with the multcomp package more or less automatically like this:> summary (glht (model, linfct = mcp (spec*comp*water = "Tukey")))Now, Mr Hothorn et al. have changed the function and it is "suggestet to the users that they write out, manually, the set of contrasts they want". I think that is not a bad idea, but I got problems with the syntax in my case. And I would really grateful if anybody could help me. So far I tried:> K <- cbind(0,diag(length(fixef(model))-1)) > rownames(K) <- names(fixef(model))[-1] > model_glht <- glht(model,linfct=K) > summary(model_glht) # but actually this is not what I wantI'm rather looking for a Tukey output like this specSt:compControl - specSt:compRoot p that they are equal < 0.05 specSt:compControl - specSt:compShoot specSt:compControl - specSt:compFull specSt:compRoot - specSt:compShoot specSt:compRoot - specSt:compFull specSt:compShoot - specSt:compFull and that for each of the 3 studied species, i.e. compare all-pairwise combinations of the levels of the second factor within each level of the first factor. We are not interested in the third factor, so data can be averaged over that. THANKS A LOT FOR ANY ADVICE, Sincerely Anna Radtke -----> model output: Linear mixed model fit by REML Formula: length ~ spec * comp * water + (meas | box_id) Data: growth AIC BIC logLik deviance REMLdev 4800 4909 -2372 4988 4744 Random effects: Groups Name Variance Std.Dev. Corr box_id (Intercept) 0.0 0.000 meas 222484.2 471.682 NaN Residual 9439.4 97.157 Number of obs: 360, groups: box_id, 120 Fixed effects: Estimate Std. Error t value (Intercept) -353.554 65.777 -5.375 specSt -103.111 93.023 -1.108 specSv -4.485 93.023 -0.048 comproot -41.686 93.023 -0.448 compshoot 241.847 93.023 2.600 compxfull 316.849 93.023 3.406 watermoist -48.620 93.023 -0.523 specSt:comproot 97.168 131.554 0.739 specSv:comproot 54.849 131.554 0.417 specSt:compshoot 10.416 131.554 0.079 specSv:compshoot 6.584 131.554 0.050 specSt:compxfull 90.949 131.554 0.691 specSv:compxfull 43.284 131.554 0.329 specSt:watermoist 9.222 131.554 0.070 specSv:watermoist 104.229 131.554 0.792 comproot:watermoist 15.931 131.554 0.121 compshoot:watermoist 28.999 131.554 0.220 compxfull:watermoist 39.363 131.554 0.299 specSt:comproot:watermoist 45.513 186.046 0.245 specSv:comproot:watermoist -24.350 186.046 -0.131 specSt:compshoot:watermoist 46.027 186.046 0.247 specSv:compshoot:watermoist 1.232 186.046 0.007 specSt:compxfull:watermoist 23.749 186.046 0.128 specSv:compxfull:watermoist -57.109 186.046 -0.307 -------------- next part -------------- "meas" "spec" "comp" "water" "box_id" "sprouts" "leaves" "length" "long.sprout" "81" 1 "Sf" "xfull" "moist" 81 6.8 13.6 150.4 43.8 "82" 1 "Sf" "root" "moist" 82 6.5 18.5 104.25 25.75 "83" 1 "Sf" "control" "moist" 83 9.25 29 146 27 "84" 1 "Sf" "control" "moist" 84 6.8 31.4 163.8 46.6 "85" 1 "Sf" "shoot" "moist" 85 4.8 19.8 127.6 32.8 "86" 1 "Sf" "control" "moist" 86 8.2 30.6 144.4 31.6 "87" 1 "Sf" "root" "moist" 87 7.8 32.8 174 38.8 "88" 1 "Sf" "shoot" "moist" 88 5 8.5 54 22.25 "89" 1 "Sf" "xfull" "moist" 89 2.25 7 54.25 27 "90" 1 "Sf" "control" "moist" 90 5.6 21.2 103 30 "91" 1 "Sf" "shoot" "moist" 91 4.25 18 84.25 29.25 "92" 1 "Sf" "root" "moist" 92 5.8 26.4 133.2 33 "93" 1 "Sf" "shoot" "moist" 93 11.25 35.25 215 44.25 "94" 1 "Sf" "root" "moist" 94 6 17.33333333 90.66666667 19.33333333 "95" 1 "Sf" "root" "moist" 95 4.666666667 5.333333333 33.66666667 9.333333333 "96" 1 "Sf" "shoot" "moist" 96 6.5 11.5 88.25 25.5 "97" 1 "Sf" "xfull" "moist" 97 9.8 13 184.4 40.2 "98" 1 "Sf" "xfull" "moist" 98 5.8 15.2 156.6 41.6 "99" 1 "Sf" "control" "moist" 99 7.5 26.5 140.75 36.5 "100" 1 "Sf" "xfull" "moist" 100 4.2 10.4 91 41.2 "101" 1 "Sf" "control" "awater-logged" 101 9 35.8 222.2 43.6 "102" 1 "Sf" "shoot" "awater-logged" 102 10.8 40.6 245.4 47.6 "103" 1 "Sf" "xfull" "awater-logged" 103 4.6 10.6 134.6 47 "104" 1 "Sf" "shoot" "awater-logged" 104 8.2 35.4 263.4 56.2 "105" 1 "Sf" "xfull" "awater-logged" 105 8.4 8 207.8 45.6 "106" 1 "Sf" "control" "awater-logged" 106 8.5 38.5 243.25 45.25 "107" 1 "Sf" "root" "awater-logged" 107 9 41 205 43.2 "108" 1 "Sf" "shoot" "awater-logged" 108 8.4 24.6 170.4 39.2 "109" 1 "Sf" "root" "awater-logged" 109 8.8 29.2 176.4 31.4 "110" 1 "Sf" "control" "awater-logged" 110 7 28.8 167 44 "111" 1 "Sf" "shoot" "awater-logged" 111 10.2 41.2 283.4 50.6 "112" 1 "Sf" "root" "awater-logged" 112 8.6 35.6 211.6 37.4 "113" 1 "Sf" "root" "awater-logged" 113 10.6 39 221 44.6 "114" 1 "Sf" "xfull" "awater-logged" 114 8 11.8 253.6 64.4 "115" 1 "Sf" "shoot" "awater-logged" 115 8.4 25 148 30.6 "116" 1 "Sf" "control" "awater-logged" 116 8.6 37.2 213 46.6 "117" 1 "Sf" "root" "awater-logged" 117 9.8 28 165.4 35.4 "118" 1 "Sf" "xfull" "awater-logged" 118 6.2 2.6 124 34 "119" 1 "Sf" "control" "awater-logged" 119 6.2 25.6 145 39.8 "120" 1 "Sf" "xfull" "awater-logged" 120 8.5 9.75 247.25 62.25 "811" 2 "Sf" "xfull" "moist" 81 4.4 41.6 464.6 217.6 "821" 2 "Sf" "root" "moist" 82 4.6 58.4 800 271.8 "831" 2 "Sf" "control" "moist" 83 7 79.2 967.8 248.8 "841" 2 "Sf" "control" "moist" 84 5.8 68.6 923 256.4 "851" 2 "Sf" "shoot" "moist" 85 4.4 46.4 549.6 263.8 "861" 2 "Sf" "control" "moist" 86 6.8 81.2 1077 309 "871" 2 "Sf" "root" "moist" 87 6.6 72.6 914.8 307.4 "881" 2 "Sf" "shoot" "moist" 88 3 28.25 361 242 "891" 2 "Sf" "xfull" "moist" 89 2.2 19.8 221.8 139.2 "901" 2 "Sf" "control" "moist" 90 5.8 72.4 1038.4 328.6 "911" 2 "Sf" "shoot" "moist" 91 4.5 39 423.25 253.25 "921" 2 "Sf" "root" "moist" 92 4.8 66.8 930.8 296.8 "931" 2 "Sf" "shoot" "moist" 93 7.75 69.5 843 323.25 "941" 2 "Sf" "root" "moist" 94 5.75 56.75 710.75 205 "951" 2 "Sf" "root" "moist" 95 6.666666667 68.33333333 703.6666667 136.3333333 "961" 2 "Sf" "shoot" "moist" 96 4.5 40.5 524.5 235.25 "971" 2 "Sf" "xfull" "moist" 97 4.2 26.4 277.2 159.8 "981" 2 "Sf" "xfull" "moist" 98 2.2 17.6 197.2 143.6 "991" 2 "Sf" "control" "moist" 99 5.8 63.6 660.4 230 "1001" 2 "Sf" "xfull" "moist" 100 2.2 24.8 252.6 179.8 "1011" 2 "Sf" "control" "awater-logged" 101 6.8 87 1356.6 346.6 "1021" 2 "Sf" "shoot" "awater-logged" 102 7.4 73.2 887 350.8 "1031" 2 "Sf" "xfull" "awater-logged" 103 3.8 42 582.4 330 "1041" 2 "Sf" "shoot" "awater-logged" 104 6.2 66.8 818.4 381.8 "1051" 2 "Sf" "xfull" "awater-logged" 105 4.6 28.4 258.8 136 "1061" 2 "Sf" "control" "awater-logged" 106 6.8 78.8 957.8 286 "1071" 2 "Sf" "root" "awater-logged" 107 6.6 85.6 1345.2 351.2 "1081" 2 "Sf" "shoot" "awater-logged" 108 5.6 61 861.6 371 "1091" 2 "Sf" "root" "awater-logged" 109 6.2 71.8 1051.6 337.2 "1101" 2 "Sf" "control" "awater-logged" 110 5.8 67.2 992.4 338 "1111" 2 "Sf" "shoot" "awater-logged" 111 7 74.4 936 362.6 "1121" 2 "Sf" "root" "awater-logged" 112 6.8 110.6 1490.4 369.8 "1131" 2 "Sf" "root" "awater-logged" 113 7.8 89 1328.6 373.8 "1141" 2 "Sf" "xfull" "awater-logged" 114 3.8 28.4 330 165.8 "1151" 2 "Sf" "shoot" "awater-logged" 115 5.8 54 647 301.2 "1161" 2 "Sf" "control" "awater-logged" 116 7 83.4 1332.2 384 "1171" 2 "Sf" "root" "awater-logged" 117 7 83.6 1212 341 "1181" 2 "Sf" "xfull" "awater-logged" 118 4.6 28.2 405.2 193.8 "1191" 2 "Sf" "control" "awater-logged" 119 5.2 70 815.4 271 "1201" 2 "Sf" "xfull" "awater-logged" 120 6 45.25 507 205.75 "812" 3 "Sf" "xfull" "moist" 81 3.4 38.4 635.6 387.8 "822" 3 "Sf" "root" "moist" 82 4.8 64.2 1163 441.2 "832" 3 "Sf" "control" "moist" 83 5.2 78.2 1470.4 481.2 "842" 3 "Sf" "control" "moist" 84 4 72.2 1401.8 499.4 "852" 3 "Sf" "shoot" "moist" 85 2.4 40.8 797.8 519.2 "862" 3 "Sf" "control" "moist" 86 4.8 80.8 1562.6 563.6 "872" 3 "Sf" "root" "moist" 87 3.4 57.8 1224.8 499.4 "882" 3 "Sf" "shoot" "moist" 88 2.6 30 565 430 "892" 3 "Sf" "xfull" "moist" 89 2 25.4 435.2 362.4 "902" 3 "Sf" "control" "moist" 90 3.8 68.4 1353.8 508.4 "912" 3 "Sf" "shoot" "moist" 91 3.25 34.5 593 465.5 "922" 3 "Sf" "root" "moist" 92 4.6 73.8 1406.4 495.8 "932" 3 "Sf" "shoot" "moist" 93 3 43.4 873.2 516.6 "942" 3 "Sf" "root" "moist" 94 5 72.75 1301.25 457 "952" 3 "Sf" "root" "moist" 95 6.333333333 90.33333333 1401.333333 350.6666667 "962" 3 "Sf" "shoot" "moist" 96 2.25 39.75 738.25 433.75 "972" 3 "Sf" "xfull" "moist" 97 3.6 35.6 428.2 281 "982" 3 "Sf" "xfull" "moist" 98 3.4 28.8 412.6 303.8 "992" 3 "Sf" "control" "moist" 99 4.8 89.4 1151.4 443.8 "1002" 3 "Sf" "xfull" "moist" 100 2.2 29.2 467.4 377 "1012" 3 "Sf" "control" "awater-logged" 101 6 83.6 1659.4 473.2 "1022" 3 "Sf" "shoot" "awater-logged" 102 3.4 56.2 1122.4 566.2 "1032" 3 "Sf" "xfull" "awater-logged" 103 2.8 46.4 882.4 633.2 "1042" 3 "Sf" "shoot" "awater-logged" 104 4 58.8 1052.8 636.4 "1052" 3 "Sf" "xfull" "awater-logged" 105 3.6 40.6 516.6 345 "1062" 3 "Sf" "control" "awater-logged" 106 4.6 71.6 1441 509.8 "1072" 3 "Sf" "root" "awater-logged" 107 5.4 92.6 1879 609 "1082" 3 "Sf" "shoot" "awater-logged" 108 2.2 46.8 1038.8 639.2 "1092" 3 "Sf" "root" "awater-logged" 109 5.4 73.6 1364.8 538.4 "1102" 3 "Sf" "control" "awater-logged" 110 4.4 80.4 1382.6 548.6 "1112" 3 "Sf" "shoot" "awater-logged" 111 3.4 67.6 1403.4 632.4 "1122" 3 "Sf" "root" "awater-logged" 112 6.4 103.6 1917.2 504.2 "1132" 3 "Sf" "root" "awater-logged" 113 5 89.8 1817.8 606 "1142" 3 "Sf" "xfull" "awater-logged" 114 2.2 27.8 424.8 347 "1152" 3 "Sf" "shoot" "awater-logged" 115 1.6 30.8 646 523.4 "1162" 3 "Sf" "control" "awater-logged" 116 5.2 84.4 1804.8 598.2 "1172" 3 "Sf" "root" "awater-logged" 117 5.8 80 1388.4 461.2 "1182" 3 "Sf" "xfull" "awater-logged" 118 3.4 42.8 630.4 341.4 "1192" 3 "Sf" "control" "awater-logged" 119 3.8 82.4 1276.8 541.6 "1202" 3 "Sf" "xfull" "awater-logged" 120 3.75 57 870.75 483.75 "1" 1 "Sv" "control" "moist" 1 8.8 37.8 211.2 60.6 "2" 1 "Sv" "xfull" "moist" 2 7 8 174.8 62.8 "3" 1 "Sv" "control" "moist" 3 9 42 255.2 66 "4" 1 "Sv" "root" "moist" 4 7.8 35 225.8 78.2 "5" 1 "Sv" "root" "moist" 5 7.8 31.4 182.6 59.4 "6" 1 "Sv" "shoot" "moist" 6 8.8 50.8 247.6 53.4 "7" 1 "Sv" "root" "moist" 7 7 26.2 169.2 63 "8" 1 "Sv" "xfull" "moist" 8 4.6 4 71.8 35.8 "9" 1 "Sv" "root" "moist" 9 8.8 40 246.8 69.4 "10" 1 "Sv" "shoot" "moist" 10 8 48.2 266.8 67.6 "11" 1 "Sv" "control" "moist" 11 15 71.8 380.2 53.6 "12" 1 "Sv" "shoot" "moist" 12 10.4 41.2 271.8 58.6 "13" 1 "Sv" "shoot" "moist" 13 8.2 44 243.8 53.4 "14" 1 "Sv" "xfull" "moist" 14 6.2 10 171.6 67.2 "15" 1 "Sv" "root" "moist" 15 9.6 42.2 187.6 57.8 "16" 1 "Sv" "xfull" "moist" 16 6.2 7.8 164 63.6 "17" 1 "Sv" "control" "moist" 17 11 53.4 262.4 59.6 "18" 1 "Sv" "control" "moist" 18 8.8 45 245.4 63.8 "19" 1 "Sv" "xfull" "moist" 19 6.2 7.4 161 61.6 "20" 1 "Sv" "shoot" "moist" 20 9.4 38.4 209 74.8 "21" 1 "Sv" "root" "awater-logged" 21 8.6 42 252 75.2 "22" 1 "Sv" "shoot" "awater-logged" 22 7.8 34.8 238.8 80.8 "23" 1 "Sv" "control" "awater-logged" 23 9.75 37.25 182.25 55.5 "24" 1 "Sv" "control" "awater-logged" 24 8.2 51 287.2 77.8 "25" 1 "Sv" "control" "awater-logged" 25 8.5 45.25 290.5 85 "26" 1 "Sv" "xfull" "awater-logged" 26 8.8 11.6 204.4 58.8 "27" 1 "Sv" "shoot" "awater-logged" 27 6.6 22 138.6 68.6 "28" 1 "Sv" "shoot" "awater-logged" 28 10.4 34.4 225.2 70 "29" 1 "Sv" "root" "awater-logged" 29 5 22.4 113.4 60.2 "30" 1 "Sv" "control" "awater-logged" 30 7.4 38 220.4 73.8 "31" 1 "Sv" "xfull" "awater-logged" 31 7.6 5 143.8 54.8 "32" 1 "Sv" "xfull" "awater-logged" 32 3.6 10.2 124.6 74.2 "33" 1 "Sv" "xfull" "awater-logged" 33 7.2 6 162.6 60.8 "34" 1 "Sv" "shoot" "awater-logged" 34 7.4 26.4 210.4 86.2 "35" 1 "Sv" "root" "awater-logged" 35 8.6 34.8 257.6 81.6 "36" 1 "Sv" "root" "awater-logged" 36 9.4 31 196.2 70.4 "37" 1 "Sv" "root" "awater-logged" 37 6.8 34.2 204.4 85.4 "38" 1 "Sv" "xfull" "awater-logged" 38 7.2 6.6 151.6 66 "39" 1 "Sv" "shoot" "awater-logged" 39 7.6 39.6 227.4 63.8 "40" 1 "Sv" "control" "awater-logged" 40 8.4 51.6 257.6 78.6 "41" 2 "Sv" "control" "moist" 1 5.4 100.8 1104.4 392.6 "42" 2 "Sv" "xfull" "moist" 2 5.2 33.6 404.4 182.4 "43" 2 "Sv" "control" "moist" 3 6.4 108.6 1060 364.2 "44" 2 "Sv" "root" "moist" 4 5 91.4 1017.6 394.4 "45" 2 "Sv" "root" "moist" 5 5.2 78.4 772.4 312.4 "46" 2 "Sv" "shoot" "moist" 6 5.6 77.6 775.6 318 "47" 2 "Sv" "root" "moist" 7 4.6 73.2 730.2 314.4 "48" 2 "Sv" "xfull" "moist" 8 3.6 17.4 238.8 136.6 "49" 2 "Sv" "root" "moist" 9 4.6 94.2 980.4 366.8 "50" 2 "Sv" "shoot" "moist" 10 7.75 96.5 876.25 327 "51" 2 "Sv" "control" "moist" 11 10 127.2 1209.6 327.2 "52" 2 "Sv" "shoot" "moist" 12 7.4 83.4 818.6 314 "53" 2 "Sv" "shoot" "moist" 13 6.8 83.8 812.4 283.6 "54" 2 "Sv" "xfull" "moist" 14 3.8 29.8 325 183.4 "55" 2 "Sv" "root" "moist" 15 4.6 72.2 734.8 378 "56" 2 "Sv" "xfull" "moist" 16 2.6 24.4 282 189 "57" 2 "Sv" "control" "moist" 17 8 124.6 1246.8 371.4 "58" 2 "Sv" "control" "moist" 18 4.4 84.4 1061.4 377.2 "59" 2 "Sv" "xfull" "moist" 19 4.2 29 353.8 193.4 "60" 2 "Sv" "shoot" "moist" 20 5.6 74.4 743.2 378.8 "61" 2 "Sv" "root" "awater-logged" 21 6.6 93.6 912.4 351.6 "62" 2 "Sv" "shoot" "awater-logged" 22 4 62 714.8 401 "63" 2 "Sv" "control" "awater-logged" 23 5 82 881.6 323.2 "64" 2 "Sv" "control" "awater-logged" 24 5.6 110.8 1264.8 424.8 "65" 2 "Sv" "control" "awater-logged" 25 5.8 89.6 930.8 346.2 "66" 2 "Sv" "xfull" "awater-logged" 26 4 39.6 455.2 268.8 "67" 2 "Sv" "shoot" "awater-logged" 27 3.4 55.4 614.6 384.2 "68" 2 "Sv" "shoot" "awater-logged" 28 5.4 71 698 320.6 "69" 2 "Sv" "root" "awater-logged" 29 3 66.4 757.4 427.8 "70" 2 "Sv" "control" "awater-logged" 30 4.8 83 990.4 413.4 "71" 2 "Sv" "xfull" "awater-logged" 31 3.2 23.2 300.6 238.8 "72" 2 "Sv" "xfull" "awater-logged" 32 1.4 24.6 210.8 192.8 "73" 2 "Sv" "xfull" "awater-logged" 33 3.2 28.6 344.8 185.8 "74" 2 "Sv" "shoot" "awater-logged" 34 3.6 59.6 631 402 "75" 2 "Sv" "root" "awater-logged" 35 5.2 86.4 826.4 289.8 "76" 2 "Sv" "root" "awater-logged" 36 7.2 87.4 918.8 351 "77" 2 "Sv" "root" "awater-logged" 37 4.2 84.8 840 422 "78" 2 "Sv" "xfull" "awater-logged" 38 2.4 29.8 355.6 239.6 "79" 2 "Sv" "shoot" "awater-logged" 39 6.2 71.2 683.8 282 "80" 2 "Sv" "control" "awater-logged" 40 5.4 106.4 1083.6 388.6 "813" 3 "Sv" "control" "moist" 1 5.2 149.6 1863.8 682 "823" 3 "Sv" "xfull" "moist" 2 2.5 42.25 427.5 344.5 "833" 3 "Sv" "control" "moist" 3 4.2 134 1496.2 572 "843" 3 "Sv" "root" "moist" 4 3.6 121 1488.8 706.8 "853" 3 "Sv" "root" "moist" 5 4 115 1340.2 587.2 "863" 3 "Sv" "shoot" "moist" 6 2.6 80 976 613.2 "873" 3 "Sv" "root" "moist" 7 3.6 94.4 1187.2 555 "883" 3 "Sv" "xfull" "moist" 8 3.5 41.75 367 195.5 "893" 3 "Sv" "root" "moist" 9 3.6 120.2 1450.4 630.6 "903" 3 "Sv" "shoot" "moist" 10 2.6 85 1007.2 586 "913" 3 "Sv" "control" "moist" 11 4 118.8 1386 626.6 "923" 3 "Sv" "shoot" "moist" 12 2.2 72.4 942.6 578.4 "933" 3 "Sv" "shoot" "moist" 13 2.2 80.6 957.6 606 "943" 3 "Sv" "xfull" "moist" 14 1.25 27 308 297 "953" 3 "Sv" "root" "moist" 15 2.6 85.8 1080.2 676.8 "963" 3 "Sv" "xfull" "moist" 16 1.666666667 41 475.6666667 374 "973" 3 "Sv" "control" "moist" 17 4.4 148 1795.6 681.8 "983" 3 "Sv" "control" "moist" 18 3.4 107.8 1484.4 653.6 "993" 3 "Sv" "xfull" "moist" 19 2.4 49.8 450.6 326.2 "1003" 3 "Sv" "shoot" "moist" 20 2.8 73.6 896.2 663.8 "1013" 3 "Sv" "root" "awater-logged" 21 4.8 119 1431.6 585 "1023" 3 "Sv" "shoot" "awater-logged" 22 1.8 61.2 896.8 654 "1033" 3 "Sv" "control" "awater-logged" 23 4.6 123.8 1471.6 552 "1043" 3 "Sv" "control" "awater-logged" 24 5 144 1906.6 734.2 "1053" 3 "Sv" "control" "awater-logged" 25 3.4 107.8 1388.4 639 "1063" 3 "Sv" "xfull" "awater-logged" 26 1.8 53.2 586.8 439.4 "1073" 3 "Sv" "shoot" "awater-logged" 27 1.8 68.4 910.6 704.4 "1083" 3 "Sv" "shoot" "awater-logged" 28 3.4 88.2 1092 615 "1093" 3 "Sv" "root" "awater-logged" 29 2.8 101.2 1342.8 740.4 "1103" 3 "Sv" "control" "awater-logged" 30 4 125.4 1556.6 769.6 "1113" 3 "Sv" "xfull" "awater-logged" 31 2 52.2 534.8 451 "1123" 3 "Sv" "xfull" "awater-logged" 32 1.8 40.4 428.6 375.6 "1133" 3 "Sv" "xfull" "awater-logged" 33 2 41.2 420.6 295.6 "1143" 3 "Sv" "shoot" "awater-logged" 34 1.8 74.6 978.8 760.6 "1153" 3 "Sv" "root" "awater-logged" 35 4 110.8 1262.8 494.4 "1163" 3 "Sv" "root" "awater-logged" 36 6.4 129.2 1518.8 590.4 "1173" 3 "Sv" "root" "awater-logged" 37 4.6 124.6 1389.8 746 "1183" 3 "Sv" "xfull" "awater-logged" 38 2 39 472.6 398.4 "1193" 3 "Sv" "shoot" "awater-logged" 39 1.6 59.8 809.4 576.4 "1203" 3 "Sv" "control" "awater-logged" 40 4.4 134.6 1622.6 664.4 "413" 1 "St" "root" "moist" 41 4.6 17.6 83 35.2 "423" 1 "St" "xfull" "moist" 42 3 4.6 29.6 15.6 "433" 1 "St" "root" "moist" 43 5.333333333 26.33333333 110.3333333 52.33333333 "443" 1 "St" "control" "moist" 44 8 38 187 63.4 "453" 1 "St" "control" "moist" 45 5.6 23.8 96.4 38.8 "463" 1 "St" "root" "moist" 46 7.5 18.75 92.25 33 "473" 1 "St" "xfull" "moist" 47 6.333333333 8 73 23 "483" 1 "St" "shoot" "moist" 48 6.4 20.6 93.4 22.4 "493" 1 "St" "xfull" "moist" 49 6 2.6 63 15 "503" 1 "St" "xfull" "moist" 50 5.4 2.8 56.4 21.2 "513" 1 "St" "shoot" "moist" 51 8 24.33333333 117 42.33333333 "523" 1 "St" "shoot" "moist" 52 7 13.4 80 25.8 "533" 1 "St" "root" "moist" 53 4.5 16 66.25 31 "543" 1 "St" "control" "moist" 54 6.8 19.4 83.8 32.6 "553" 1 "St" "control" "moist" 55 9.25 36.75 158.5 34.25 "563" 1 "St" "control" "moist" 56 5.2 18.2 97.6 39 "573" 1 "St" "shoot" "moist" 57 6.25 12.75 55.75 25 "583" 1 "St" "shoot" "moist" 58 6.333333333 30.66666667 131.3333333 39 "593" 1 "St" "xfull" "moist" 59 5.8 7.8 69.2 28.6 "603" 1 "St" "root" "moist" 60 5 19.8 84 27.6 "613" 1 "St" "xfull" "awater-logged" 61 6.2 3.2 73.4 21 "623" 1 "St" "control" "awater-logged" 62 7.2 15 89.2 32 "633" 1 "St" "shoot" "awater-logged" 63 6.5 15.25 85.25 35 "643" 1 "St" "root" "awater-logged" 64 4.666666667 18.66666667 99 40.33333333 "653" 1 "St" "control" "awater-logged" 65 5.8 16.8 75.8 26.2 "663" 1 "St" "xfull" "awater-logged" 66 5.666666667 4 62.33333333 28.33333333 "673" 1 "St" "control" "awater-logged" 67 9 33.25 120 37 "683" 1 "St" "root" "awater-logged" 68 6 16 70 24.25 "693" 1 "St" "shoot" "awater-logged" 69 7 15.4 71.6 31.4 "703" 1 "St" "xfull" "awater-logged" 70 5.2 1.2 57.4 23.6 "713" 1 "St" "control" "awater-logged" 71 7.25 23.5 114 33 "723" 1 "St" "shoot" "awater-logged" 72 6.75 30.5 125 40.75 "733" 1 "St" "shoot" "awater-logged" 73 7.25 13.75 81.5 29.5 "743" 1 "St" "root" "awater-logged" 74 8.2 18.2 89.8 35.6 "753" 1 "St" "shoot" "awater-logged" 75 5.6 15.8 69.6 29.4 "763" 1 "St" "control" "awater-logged" 76 7.4 27.8 100.2 28.8 "773" 1 "St" "root" "awater-logged" 77 6.4 15.4 79.2 36 "783" 1 "St" "xfull" "awater-logged" 78 6.2 0.6 42.6 13.4 "793" 1 "St" "xfull" "awater-logged" 79 3.8 3.8 46.4 22.6 "803" 1 "St" "root" "awater-logged" 80 5.2 26 92.4 25.6 "411" 2 "St" "root" "moist" 41 3 45.6 658.4 364.8 "421" 2 "St" "xfull" "moist" 42 3.4 9.2 130 93.2 "431" 2 "St" "root" "moist" 43 3.25 36 520.75 382.25 "441" 2 "St" "control" "moist" 44 5.6 68.2 966.2 456.4 "451" 2 "St" "control" "moist" 45 4.8 60.4 765.4 347.8 "461" 2 "St" "root" "moist" 46 6.8 57.2 631.4 282.8 "471" 2 "St" "xfull" "moist" 47 4.8 10 133.8 50.8 "481" 2 "St" "shoot" "moist" 48 6.6 61.2 547.4 233.6 "491" 2 "St" "xfull" "moist" 49 7.6 24.6 241.2 88 "501" 2 "St" "xfull" "moist" 50 5.4 19.4 138.2 43 "511" 2 "St" "shoot" "moist" 51 5 42.4 353.8 188 "521" 2 "St" "shoot" "moist" 52 4.6 39.2 417 256 "531" 2 "St" "root" "moist" 53 3.75 51 607.75 298.25 "541" 2 "St" "control" "moist" 54 6.2 66.8 771.4 303.4 "551" 2 "St" "control" "moist" 55 7 79 998 398 "561" 2 "St" "control" "moist" 56 4.6 58.8 782.4 365 "571" 2 "St" "shoot" "moist" 57 4.4 37.6 331 143 "581" 2 "St" "shoot" "moist" 58 4.25 44.25 466.5 261.25 "591" 2 "St" "xfull" "moist" 59 6.6 18.8 160.6 64.6 "601" 2 "St" "root" "moist" 60 3.8 49 668.8 311 "611" 2 "St" "xfull" "awater-logged" 61 6 17.8 204.2 96.4 "621" 2 "St" "control" "awater-logged" 62 4.2 56.8 800.4 353.6 "631" 2 "St" "shoot" "awater-logged" 63 3.5 45 534 305 "641" 2 "St" "root" "awater-logged" 64 4.666666667 61.33333333 665.6666667 338.6666667 "651" 2 "St" "control" "awater-logged" 65 4.2 58.8 759 323.2 "661" 2 "St" "xfull" "awater-logged" 66 4.5 14.25 171 97 "671" 2 "St" "control" "awater-logged" 67 5.5 72 851.5 363 "681" 2 "St" "root" "awater-logged" 68 5.75 62.75 616.75 262.25 "691" 2 "St" "shoot" "awater-logged" 69 3.2 41.6 587.8 415.4 "701" 2 "St" "xfull" "awater-logged" 70 3.8 14 141.4 79.8 "711" 2 "St" "control" "awater-logged" 71 4.2 57 774 298.2 "721" 2 "St" "shoot" "awater-logged" 72 3.8 41.4 468.4 227.2 "731" 2 "St" "shoot" "awater-logged" 73 3.75 42.25 492.25 235 "741" 2 "St" "root" "awater-logged" 74 4.4 52.4 691.2 342 "751" 2 "St" "shoot" "awater-logged" 75 2.4 30.6 379.4 294.2 "761" 2 "St" "control" "awater-logged" 76 6.6 80.2 966.6 340.6 "771" 2 "St" "root" "awater-logged" 77 3.4 38 526.8 361.2 "781" 2 "St" "xfull" "awater-logged" 78 4.6 17.4 198.2 107.4 "791" 2 "St" "xfull" "awater-logged" 79 3 14.2 166.6 104.4 "801" 2 "St" "root" "awater-logged" 80 5.4 63.8 636.6 292.6 "412" 3 "St" "root" "moist" 41 2.6 60.6 1161 617.6 "422" 3 "St" "xfull" "moist" 42 3.4 16.8 313.8 250.6 "432" 3 "St" "root" "moist" 43 2.75 46 906 707.25 "442" 3 "St" "control" "moist" 44 3 74.8 1550.4 742.4 "452" 3 "St" "control" "moist" 45 3.8 73 1324.6 627.6 "462" 3 "St" "root" "moist" 46 4.4 62.2 1096 496.4 "472" 3 "St" "xfull" "moist" 47 3.2 13.8 133.6 64.4 "482" 3 "St" "shoot" "moist" 48 3.2 53 776.8 447.2 "492" 3 "St" "xfull" "moist" 49 3.8 21.4 303.4 239.6 "502" 3 "St" "xfull" "moist" 50 7.5 18.75 207.5 58 "512" 3 "St" "shoot" "moist" 51 4.6 56.8 689 421.8 "522" 3 "St" "shoot" "moist" 52 1.6 34.6 640.4 575.8 "532" 3 "St" "root" "moist" 53 3.75 67.5 1164.25 586.75 "542" 3 "St" "control" "moist" 54 3 73.4 1325 617.2 "552" 3 "St" "control" "moist" 55 4.75 99 1730.75 678 "562" 3 "St" "control" "moist" 56 3.6 75.4 1386.6 713.8 "572" 3 "St" "shoot" "moist" 57 2.25 31 463.5 343 "582" 3 "St" "shoot" "moist" 58 2.5 47 799 551.5 "592" 3 "St" "xfull" "moist" 59 5.5 14.5 243 155 "602" 3 "St" "root" "moist" 60 2.8 55.6 1086.6 513 "612" 3 "St" "xfull" "awater-logged" 61 5.4 27 345 220 "622" 3 "St" "control" "awater-logged" 62 4 80.4 1154.4 496.2 "632" 3 "St" "shoot" "awater-logged" 63 2.25 50.5 835.5 425.25 "642" 3 "St" "root" "awater-logged" 64 4.333333333 83.33333333 1354.333333 551.6666667 "652" 3 "St" "control" "awater-logged" 65 4 71.4 1351.4 576.4 "662" 3 "St" "xfull" "awater-logged" 66 3.75 17 224.5 160.25 "672" 3 "St" "control" "awater-logged" 67 4.25 78.5 1268.5 549.75 "682" 3 "St" "root" "awater-logged" 68 5.25 92.75 1189.5 462 "692" 3 "St" "shoot" "awater-logged" 69 2.2 45 865.6 642.8 "702" 3 "St" "xfull" "awater-logged" 70 3.6 22.4 246.2 181.6 "712" 3 "St" "control" "awater-logged" 71 3.8 74.6 1284 545.4 "722" 3 "St" "shoot" "awater-logged" 72 2.2 42.2 647 405.2 "732" 3 "St" "shoot" "awater-logged" 73 2.25 47.25 798.75 491.75 "742" 3 "St" "root" "awater-logged" 74 3 61 1105.8 551.4 "752" 3 "St" "shoot" "awater-logged" 75 1.8 36.6 585.8 534.4 "762" 3 "St" "control" "awater-logged" 76 5.8 98 1633.8 640.4 "772" 3 "St" "root" "awater-logged" 77 3.4 51.6 876.8 572.6 "782" 3 "St" "xfull" "awater-logged" 78 4.8 25.6 295.6 175.6 "792" 3 "St" "xfull" "awater-logged" 79 2.6 21.4 295.2 228.2 "802" 3 "St" "root" "awater-logged" 80 4 72.2 1072.8 576
Anna Radtke
2010-Oct-29 16:14 UTC
[R] Tukey post hoc comparison (glht?) after 3factorial mixed model (lmer)
Hello, dear R-community. This is a question about TukeyHSD between factor combinations of a Three-Way ANOVA, which is - since it is a multi measure ANOVA - not a simple ANOVA but a Generalised Linear Mixed Model (GLMM), calculated with "lmer".> growth <-groupedData(length~meas|box_id,outer=~spec*comp*water,data=all.spec)> model <- lmer(length~spec*comp*water+(meas|box_id),data=growth) > summary(model)This works fine. But now, I would like to calculate Tukey HSD among certain factor combinations. Formerly (before version 1.0), Tukey contrasts including all levels of interactions could be calculated with the multcomp package more or less automatically like this:> summary (glht (model, linfct = mcp (spec*comp*water = "Tukey")))Now, Mr Hothorn et al. have changed the function and it is "suggestet to the users that they write out, manually, the set of contrasts they want". I think that is not a bad idea, but I got problems with the syntax in my case. And I would really grateful if anybody could help me. So far I tried:> K <- cbind(0,diag(length(fixef(model))-1)) > rownames(K) <- names(fixef(model))[-1] > model_glht <- glht(model,linfct=K) > summary(model_glht) # but actually this is not what I wantI'm rather looking for a Tukey output like this specSt:compControl - specSt:compRoot p that they are equal < 0.05 specSt:compControl - specSt:compShoot specSt:compControl - specSt:compFull specSt:compRoot - specSt:compShoot specSt:compRoot - specSt:compFull specSt:compShoot - specSt:compFull and that for each of the 3 studied species, i.e. compare all-pairwise combinations of the levels of the second factor within each level of the first factor. We are not interested in the third factor, so data can be averaged over that. THANKS A LOT FOR ANY ADVICE, Sincerely Anna Radtke -----> model output: Linear mixed model fit by REML Formula: length ~ spec * comp * water + (meas | box_id) Data: growth AIC BIC logLik deviance REMLdev 4800 4909 -2372 4988 4744 Random effects: Groups Name Variance Std.Dev. Corr box_id (Intercept) 0.0 0.000 meas 222484.2 471.682 NaN Residual 9439.4 97.157 Number of obs: 360, groups: box_id, 120 Fixed effects: Estimate Std. Error t value (Intercept) -353.554 65.777 -5.375 specSt -103.111 93.023 -1.108 specSv -4.485 93.023 -0.048 comproot -41.686 93.023 -0.448 compshoot 241.847 93.023 2.600 compxfull 316.849 93.023 3.406 watermoist -48.620 93.023 -0.523 specSt:comproot 97.168 131.554 0.739 specSv:comproot 54.849 131.554 0.417 specSt:compshoot 10.416 131.554 0.079 specSv:compshoot 6.584 131.554 0.050 specSt:compxfull 90.949 131.554 0.691 specSv:compxfull 43.284 131.554 0.329 specSt:watermoist 9.222 131.554 0.070 specSv:watermoist 104.229 131.554 0.792 comproot:watermoist 15.931 131.554 0.121 compshoot:watermoist 28.999 131.554 0.220 compxfull:watermoist 39.363 131.554 0.299 specSt:comproot:watermoist 45.513 186.046 0.245 specSv:comproot:watermoist -24.350 186.046 -0.131 specSt:compshoot:watermoist 46.027 186.046 0.247 specSv:compshoot:watermoist 1.232 186.046 0.007 specSt:compxfull:watermoist 23.749 186.046 0.128 specSv:compxfull:watermoist -57.109 186.046 -0.307 -------------- next part -------------- "meas" "spec" "comp" "water" "box_id" "sprouts" "leaves" "length" "long.sprout" "81" 1 "Sf" "xfull" "moist" 81 6.8 13.6 150.4 43.8 "82" 1 "Sf" "root" "moist" 82 6.5 18.5 104.25 25.75 "83" 1 "Sf" "control" "moist" 83 9.25 29 146 27 "84" 1 "Sf" "control" "moist" 84 6.8 31.4 163.8 46.6 "85" 1 "Sf" "shoot" "moist" 85 4.8 19.8 127.6 32.8 "86" 1 "Sf" "control" "moist" 86 8.2 30.6 144.4 31.6 "87" 1 "Sf" "root" "moist" 87 7.8 32.8 174 38.8 "88" 1 "Sf" "shoot" "moist" 88 5 8.5 54 22.25 "89" 1 "Sf" "xfull" "moist" 89 2.25 7 54.25 27 "90" 1 "Sf" "control" "moist" 90 5.6 21.2 103 30 "91" 1 "Sf" "shoot" "moist" 91 4.25 18 84.25 29.25 "92" 1 "Sf" "root" "moist" 92 5.8 26.4 133.2 33 "93" 1 "Sf" "shoot" "moist" 93 11.25 35.25 215 44.25 "94" 1 "Sf" "root" "moist" 94 6 17.33333333 90.66666667 19.33333333 "95" 1 "Sf" "root" "moist" 95 4.666666667 5.333333333 33.66666667 9.333333333 "96" 1 "Sf" "shoot" "moist" 96 6.5 11.5 88.25 25.5 "97" 1 "Sf" "xfull" "moist" 97 9.8 13 184.4 40.2 "98" 1 "Sf" "xfull" "moist" 98 5.8 15.2 156.6 41.6 "99" 1 "Sf" "control" "moist" 99 7.5 26.5 140.75 36.5 "100" 1 "Sf" "xfull" "moist" 100 4.2 10.4 91 41.2 "101" 1 "Sf" "control" "awater-logged" 101 9 35.8 222.2 43.6 "102" 1 "Sf" "shoot" "awater-logged" 102 10.8 40.6 245.4 47.6 "103" 1 "Sf" "xfull" "awater-logged" 103 4.6 10.6 134.6 47 "104" 1 "Sf" "shoot" "awater-logged" 104 8.2 35.4 263.4 56.2 "105" 1 "Sf" "xfull" "awater-logged" 105 8.4 8 207.8 45.6 "106" 1 "Sf" "control" "awater-logged" 106 8.5 38.5 243.25 45.25 "107" 1 "Sf" "root" "awater-logged" 107 9 41 205 43.2 "108" 1 "Sf" "shoot" "awater-logged" 108 8.4 24.6 170.4 39.2 "109" 1 "Sf" "root" "awater-logged" 109 8.8 29.2 176.4 31.4 "110" 1 "Sf" "control" "awater-logged" 110 7 28.8 167 44 "111" 1 "Sf" "shoot" "awater-logged" 111 10.2 41.2 283.4 50.6 "112" 1 "Sf" "root" "awater-logged" 112 8.6 35.6 211.6 37.4 "113" 1 "Sf" "root" "awater-logged" 113 10.6 39 221 44.6 "114" 1 "Sf" "xfull" "awater-logged" 114 8 11.8 253.6 64.4 "115" 1 "Sf" "shoot" "awater-logged" 115 8.4 25 148 30.6 "116" 1 "Sf" "control" "awater-logged" 116 8.6 37.2 213 46.6 "117" 1 "Sf" "root" "awater-logged" 117 9.8 28 165.4 35.4 "118" 1 "Sf" "xfull" "awater-logged" 118 6.2 2.6 124 34 "119" 1 "Sf" "control" "awater-logged" 119 6.2 25.6 145 39.8 "120" 1 "Sf" "xfull" "awater-logged" 120 8.5 9.75 247.25 62.25 "811" 2 "Sf" "xfull" "moist" 81 4.4 41.6 464.6 217.6 "821" 2 "Sf" "root" "moist" 82 4.6 58.4 800 271.8 "831" 2 "Sf" "control" "moist" 83 7 79.2 967.8 248.8 "841" 2 "Sf" "control" "moist" 84 5.8 68.6 923 256.4 "851" 2 "Sf" "shoot" "moist" 85 4.4 46.4 549.6 263.8 "861" 2 "Sf" "control" "moist" 86 6.8 81.2 1077 309 "871" 2 "Sf" "root" "moist" 87 6.6 72.6 914.8 307.4 "881" 2 "Sf" "shoot" "moist" 88 3 28.25 361 242 "891" 2 "Sf" "xfull" "moist" 89 2.2 19.8 221.8 139.2 "901" 2 "Sf" "control" "moist" 90 5.8 72.4 1038.4 328.6 "911" 2 "Sf" "shoot" "moist" 91 4.5 39 423.25 253.25 "921" 2 "Sf" "root" "moist" 92 4.8 66.8 930.8 296.8 "931" 2 "Sf" "shoot" "moist" 93 7.75 69.5 843 323.25 "941" 2 "Sf" "root" "moist" 94 5.75 56.75 710.75 205 "951" 2 "Sf" "root" "moist" 95 6.666666667 68.33333333 703.6666667 136.3333333 "961" 2 "Sf" "shoot" "moist" 96 4.5 40.5 524.5 235.25 "971" 2 "Sf" "xfull" "moist" 97 4.2 26.4 277.2 159.8 "981" 2 "Sf" "xfull" "moist" 98 2.2 17.6 197.2 143.6 "991" 2 "Sf" "control" "moist" 99 5.8 63.6 660.4 230 "1001" 2 "Sf" "xfull" "moist" 100 2.2 24.8 252.6 179.8 "1011" 2 "Sf" "control" "awater-logged" 101 6.8 87 1356.6 346.6 "1021" 2 "Sf" "shoot" "awater-logged" 102 7.4 73.2 887 350.8 "1031" 2 "Sf" "xfull" "awater-logged" 103 3.8 42 582.4 330 "1041" 2 "Sf" "shoot" "awater-logged" 104 6.2 66.8 818.4 381.8 "1051" 2 "Sf" "xfull" "awater-logged" 105 4.6 28.4 258.8 136 "1061" 2 "Sf" "control" "awater-logged" 106 6.8 78.8 957.8 286 "1071" 2 "Sf" "root" "awater-logged" 107 6.6 85.6 1345.2 351.2 "1081" 2 "Sf" "shoot" "awater-logged" 108 5.6 61 861.6 371 "1091" 2 "Sf" "root" "awater-logged" 109 6.2 71.8 1051.6 337.2 "1101" 2 "Sf" "control" 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