Hello all, I am trying to perform ANOVA on my sample data given below to see if any gene(column 1 stands for gene names) is differentially expressed after subjecting it to the 6 different experiments(columns 2 to 7 are experiments). Gene 14A_U133A_Detection 14B_U133A_Signal 88A_U133A_Signal 88B_U133A_Signal 183A_U133A_Signal 183B_U133A_Signal AFFX-BioB-5_at 403 409.3 611.5 569.2 536.6 580.2 AFFX-BioB-M_at 757.3 574.4 826.7 595.3 755.2 956 AFFX-BioB-3_at 284.4 327.3 421.6 336.6 391.3 412.6 AFFX-BioC-5_at 2314.2 1685.3 2264.7 2204.1 2233.1 2458.4 AFFX-BioC-3_at 1574.5 1273 1484.6 1321.2 1474.7 1774.1 AFFX-BioDn-5_at 2333.7 1796.8 2464.5 2372.5 2095.9 2735.7 AFFX-BioDn-3_at 13673.9 11463.9 13624.7 14513.9 12934.1 16293.1 AFFX-CreX-5_at 17778.8 15248.8 19977.2 19613.4 18609.1 18988.2 AFFX-CreX-3_at 31056.6 24869.9 30773.4 32918.6 34412.1 33954.6 I have to check if each gene is behaving similarly in all its experiments or if it is showing some variation in a particular experiment. I am new to R and i am not able to figure out how to do this. Thanks, Vasu. [[alternative HTML version deleted]]