Dear Colleague,
Happy New Year!
As we know, codon preference among different species could be dramatically
different. To enhance the expression level of a foreign protein in a particular
expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very
important to adjust the codon frequency of the foreign protein to match that of
the host expression system. One classic example is GFP (green fluorescent
protein) which was optimized to achieve high-level of expression in mammalian
cells.
GenScript has developed a proprietary algorithm for codon optimization. This
algorithm can optimize sequences for protein expression using either your own
codon usage table or those from publicly available codon usage database. It can
converts your amino acid sequence into a DNA sequence with overall codon usage
similar to a specified organism, and also optimizes the RNA secondary structure,
GC content, repetitive codons etc. Using Genscript optimized synthetic genes,
many of our customers have reported dramatic increase on protein expression.
The optimized gene can be synthesized by GenScript gene synthesis technology
with 100% fidelity. The cost is as low as $2.35 per base pair. Our service is
very flexible, we can clone the optimized gene into our standard vectors
(without extra charge), or any expression vector that you provide (for $400
extra charge).
Gene synthesis is a powerful technology and it has many other applications.
Another application example is to replace PCR Cloning. Please visit our web
(http://www.genscript.com/gene_synthesis.html) to learn more about this
technology.
Besides Gene Synthesis, we also provide custom vector-based siRNA, siRNA
cassette, peptide, oligo, cloning and protein expression, biochemical reagents,
and labwares. Please visit our web site (http://www.genscript.com) to learn
more about our services.
Sincerely,
Sally Wang
Account Manager
GenScript Corporation
120 Centennial Ave.
Piscataway, New Jersey 08854, USA
Tel: 1-732-885-9188, 1-732-357-3839
Fax: 1-732-210-0262
Email: sallyw at genscript.com
Web: http://www.genscript.com
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