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Dear Colleague, Happy New Year! As we know, codon preference among different species could be dramatically different. To enhance the expression level of a foreign protein in a particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very important to adjust the codon frequency of the foreign protein to match that of the host expression system. One classic example is GFP (green fluorescent protein) which was optimized to achieve high-level of expression in mammalian cells. GenScript has developed a proprietary a...

Dear Colleague, Happy New Year! As we know, codon preference among different species could be dramatically different. To enhance the expression level of a foreign protein in a particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very important to adjust the codon frequency of the foreign protein to match that of the host expression system. One classic example is GFP (green fluorescent protein) which was optimized to achieve high-level of expression in mammalian cells. GenScript has developed a proprietary a...

...available to it from the operating system. Our machine has 128G. I also use options(expressions = 100000) but I still get the above errors. Can anyone help? Im I trying to change the wrong thing or is there anything else I can do? Many thanks, Michy -- Michelle Simon Bioinformatics, MRC Mammalian Genetics Unit, Harwell, Oxon, ox11 0rd, U.K. E-mail: m.simon at har.mrc.ac.uk Telephone: +44 (0)1235 841031 www: http://www.har.mrc.ac.uk/ This email may have a PROTECTIVE MARKING, for an explanation please see: http://www.mrc.ac.uk/About/Informationandstandards/Documentmarking/index.htm