R help - Jul 2005 - How learn a probabilities matrix from a large fasta file in R?

Hi everybody,
I have a large fasta file(15M) which contains a lot of DNA sequence in fasta 
format.And i want to get probabilities matrix for 2nd order markov china 
from this background.Here is a web tool, 
http://tandem.bu.edu/markov.html.But i can not upload a large file. I wonder 
if there is a R packages can do this ,Thanks in advance .

Jiantao Shi

** <http://tandem.bu.edu/markov.html>**
<http://tandem.bu.edu/markov.html>

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