Hi all, I used R (vegan package) to make rarefaction curves and I calculated the Chao index for each curve. However, the plateau is far from reached. What I want to do now is the following: Based on the Chao index, I want to extrapolate the curve so I get an x-value which gives me an estimation of the total number of clones I'd have to pick up and sequence in order to have a full coverage of the species diversity in my sample. Is there anyone who knows how to do this? Using vegan or any other package? Or is there any other way to calculate this x-value? Thanks in advance! Guillaume -- View this message in context: http://r.789695.n4.nabble.com/Extrapolation-of-rarefaction-curve-tp4693693.html Sent from the R help mailing list archive at Nabble.com.