Dear R-helpers, I have time series data from 16 subjects and 2 treatment groups. The seasonal variation can be best described by two harmonics, I called the frequencies omega and omega2. I now want to test whether (1) the seasonal pattern differs between the treatments (some kind of overall test). If this is the case, (2) I want to conduct tests to find out whether the amplitude of the fist harmonic, the amplitude of the second harmonic, the phase of the first harmonic and the phase of the second harmonic differs between the treatment groups. My attempt was to create a lme containing the two oscillations and the treatment group: omegaDay=omega*Day # there is probably a more elegant solution omega2Day=omega2*Day m=lme(y~(cos(omegaDay)+sin(omegaDay)+cos(omega2Day)+sin(omega2Day))*treatment, random=~1|subject) summary(m) The summary table tells me that the respective elements are significant (except for cos(omegaDay):treatmentTRUE). I know how to calculate the amplitudes and phases for both treatment groups from the model estimates, but how do I know if they are significantly different? Thanks to anyone who takes the time to help me with this problem! Karin _________________________________________________________________ Hotmail: Vertrauenswürdige E-Mails dank leistungsstarkem SPAM-Schutz. https://signup.live.com/signup.aspx?id=60969 [[alternative HTML version deleted]]