Hi, I'm having difficulty to replicate in R a nested (hierarchical) anova example found in p. 308 of Zar, J.H. 1996. Bostatistical Analysis. Prentice Hall. 3rd ed. The example (15.1) is as follows: The variable is blood cholesterol concentration in women (in mg/100 ml of plasma). This variable was measured after the administration of one of three different drugs, each drug having been obtained from two sources DRUG 1 DRUG 2 DRUG 3 A Q D B L S 102 103 108 1091 104 105 104 104 110 1082 106 107 Zar results are these: ---------------------------------------------------------------------------- Source of variation SS DF MS F P ---------------------------------------------------------------------------- *Total 71.67 11 *Among all subgroups 62.67 5 *Groups 61.17 2 30.58 61.16 0.0037 *Subgroups 1.50 3 0.50 0.33 0.80 *Error 9.00 6 1.50 ---------------------------------------------------------------------------- It is straightforward to follow the equations from the book step by step to obtain those results. Now, when trying the 'aov()' function in R: ## creating the data frame zar <- data.frame( cChol=c(102, 104, 103, 104, 108, 110, 109, 108, 104, 106, 105, 107), drug=gl(3, 4, 12), dsource=gl(6, 2, 12, labels=c("A", "Q", "D", "B", "L", "S")) ) ## This gives the relevant SS, DF and MS values, but only the F and P corresponding to drug ## effect (Zar's Groups) summary( aov(cChol ~ drug + Error(dsource/drug), data=zar) ) ## The F and P values corresponding to subgroups can be obtained by: 0.5/1.5 # F pf(0.5/1.5, 3, 6, lower.tail=FALSE) # P ## or from the second row in the two way anova: summary( aov(cChol ~ drug + dsource, data=zar) ) In order to avoid confussion in our students (and myself!) I wonder if we can obtain the desired results in one step? Thank you in advance for your kind help. Héctor -- Héctor Villalobos <hvillalo@ipn.mx> CICIMAR - IPN A.P. 592. Col. Centro La Paz, Baja California Sur, MÉXICO. 23000 Tels. (+52 612) 122 53 44; 123 46 58; 123 47 34 ext. 82425 Fax. (+52 612) 122 53 22 [[alternative HTML version deleted]]