Hi,all, I have a problem about launching SJava in R from external application (Cytoscape). My R version is 2.1.0 and Sjava version is 0.68. R is installed by issuing 1. configure --enable-R-shlib 2. make 3. make check 4. make install SJava is installed by issuing "R CMD INSTALL -c SJava_version_.tar.gz". The LD_LIBRARY_PATH is set in .bash_profile by "LD_LIBRARY_PATH=${LD_LIBRARY_PATH}:/usr/local/lib/R/library/SJava/libs:/usr/java/j2sdk1.4.2_08/jre/lib/i386/client:/usr/java/j2sdk1.4.2_08/jre/lib/i386:/usr/java/j2sdk1.4.2_08/jre/../lib/i386: export LD_LIBRARY_PATH" After installation, type the following command * library(SJava) * .JavaInit() * .Java("java.lang.System", "getProperty", "java.class.path") * names(.Java("java.lang.System", "getProperties")) Get the correct output (omitted here) . Now the question is: I am using R to interact with Cytoscape ( a visualization tool), which has a plugin called "Cytotalk" that can let R and Cytoscape talk to each other. I successfully ran a R script which need SJava and got the right output in Cytoscape. But when I tried to run the R script from the cytoscape menu ( the plugin enabled a menu in which you can choose a R file to execute it and display the output in Cytoscape ), the terminal give me the following error: Accepting clients from localhost> port <- 8082 > library(SJava)Error in dyn.load(x, as.logical(local), as.logical(now)) : unable to load shared library '/usr/local/lib/R/library/SJava/libs/SJava.so': libRSNativeJava.so: cannot open shared object file: No such file or directory Error in library(SJava) : .First.lib failed for 'SJava' Execution halted RESULT: 1 I googled around and found it was a common error when people trying to install SJava. But I installed SJava correctly and it seems work fine, I can not figure out why it comes up with this error. To give you some background information, I guess Cytoscape and R talk with each other via various java stuff. The fact that I can interact with Cytoscape from within R showing that the .jar directory should be fine. The first few lines of the R code is like : library(SJava) #Warning message: #The Java machine is no longer initialized automatically. You must explicitly load it in: f(libname, pkgname) a=javaConfig(classPath="/usr/local/bio/cytoscape/plugins/cytoTalk.jar") .JavaInit(config=a) #.Java("System","getProperty","java.class.path") #[1] ":/usr/local/bio/cytoscape/plugins/cytoTalk.jar:/usr/local/lib/R/library/SJava/org/omegahat/Jars/Environment.jar:/usr/local/lib/R/library/SJava/org/..:/usr/local/lib/R/library/SJava/org/omegahat/Jars/antlr.jar:/usr/local/lib/R/library/SJava/org/omegahat/Jars/jas.jar:/usr/local/lib/R/library/SJava/org/omegahat/Jars/jhall.jar" source("CytoTalkClient-v2.R") cy <- .JNew("CytoTalkClient","http://localhost:8082") And by the way, my OS is Redhat Linux. I really appreciate it if someone has an clue of the problem. Thanks. Xiao