Dear all, I was trying to do some contrasts within ANOVA. I searched the archive and found a clue posted by Steffen Katzner ( http://tolstoy.newcastle.edu.au/R/help/06/01/19385.html) I have three levels for a factor names "StdLot". I just want to make three comparisons, 1 vs 2, 1 vs 3 and 2 vs 3. First, contrasts(d3$StdLot,3)<-matrix(c(1,-1, 0,0,1,-1,1,0,-1),3,3) #d3 is the data set. set up the contrast matrix Second, aov(Bkg~StdLot,na.rm=T,data=d3,contrasts=contrasts(d3$StdLot))->mod.aov #ANOVA, Finally, summary(mod.aov,split=list(StdLot=list('1 vs 2'=1,'2 vs 3'=2,'1 vs 3'=3))) #comparison summary Here is the final result I got, the third comparison is missing. Does anyone have any idea what is wrong here? If I change the order of the comparisons it's always the third one missing. So I guess it's not due to the data. Appreciate any comment. Df Sum Sq Mean Sq F value Pr(>F) StdLot 2 1.905 0.953 10.3769 3.710e-05 *** StdLot: 1 vs 2 1 0.223 0.223 2.4239 0.1200 StdLot: 2 vs 3 1 1.683 1.683 18.3299 2.162e-05 *** StdLot: 1 vs 3 1 Residuals 601 55.173 0.092 --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 2 observations deleted due to missingness -- Jun Shen PhD PK/PD Scientist BioPharma Services Millipore Corporation 15 Research Park Dr. St Charles, MO 63304 Direct: 636-720-1589 [[alternative HTML version deleted]]