search for: fluorescence

Hello R-help I have the following data for a standard curve concentration(nM),fluorescence 0,48.34 2,58.69 5,70.83 10,94.73 20,190.8 50,436.0 100, 957.9   (1)Is there function in R to plot a spline. (2)How can I interpolation,say 1000 point from 0nM-100nM and store this as a data frame of concentration,fluorescence (3)How can I modify the code below so that instead of retrieving a conce...

Dear All, I am doing experiments in live plant tissue using a laser confocal microscope. The method is called "fluorescence recovery after photo-bleaching" (FRAP) and here follows a short summary: 1. Record/ measure fluorescence intensity in a defined, round region of interest (ROI, in this case a small spot) to determine the initial intensity value before the bleaching. This pre-bleach value is also used for nor...

...advance): I need to analisy a long list of data time \fluoscence I want to make one plot with standard deviation and the average data... Could you suggest me how to make that.. The data have this distribution: How can read and extrapolate this data? pippo.csv ps <- read.table(pippo.csv) time fluorescence 0; 0 4.013; 62.96 6.053 ;112.3 24.14; 106.2 ... How can use a plot with standard deviation of all data: time versus fluorescen

R-help, I have the following file I want to import to R (some lines removed) Calibrated CTD data for station:00280001 Calibrated:23/8 2001, Salinity Unsmoothed, Fluorescence Uncalibrated Maximum observed depth: 36 m QUAL has one digit for each of pressure, temp., sal. and fluor. QUAL=1:Uncal., QUAL=2:OK, QUAL=6:Interp., QUAL=9:No data DEPTH CTDPRS CTDTMP CTDSAL RAWFLU NUMB. QUAL M DBAR IPTS-68...

On 6/5/2015 1:33 PM, Michael Hennebry wrote: > It's a desktop in an old house. > The outlets have ground-fault protection, > but the third prong is ungrounded. > not sure how GFI would function at all without a valid ground, unless the GFI is wired to neutral, which is dangerous on its own. you might get a 3-prong-to-2-prong adapter and plug the PC into that, leaving the ground

Hi all, excuse me by this elementary question. I wish to know if a package in language R exists to analyze FCS (Fluorescence Correlation Spectroscopy) datas. And, if it possible, in addition can read the archives in raw format generated by the ConfoCor2 program. Thanks Horacio. **************************************************************************** Dr. Horacio Castellini http://ar.geocities.com/horaci...

Hi all. I'd like know what is the format file saved by Leica Microsystems TCS SP2-AOBS equipped with a SP2-FCS2 Leica Microsystems workstation its datas. Cause it save in *.fcs extention file but ins't flow cytometry standart format file... Tahnks Horacio.

I haven't been able to fully make sense of the conflicting online information about whether and how to specify nesting structure for a nested, mixed model. I'll describe my experiment and hopefully somebody who knows lme4 well can help. We're measuring the fluorescence intensity of brain slices from frogs that have undergone various treatments. We want to use multcomp to look for differences between treatments, while accounting for the variance introduced by the random effects of brain and slice. There are a few measurements per slice, several slices per brain,...

Hi, Can anyone shed some light on this: My PC's are on a separate power circuit. Each PC has its own UPS. I have an old Honda generator. When the power goes out, I fire up the generator. However, the UPS's still run on battery. If I connect the PC directly to the socket and bypass the UPS, the PC works. I was told by a UPS supplier, that the power output from the generator was

Hello everyone, I have come across a bit of an odd problem: I am currently analysing data PCR reaction data of which part is behaving exponential. I would like to fit the exponential part to the following: y0 + alpha * E^t In which Y0 is the groundphase, alpha a fluorescence factor, E the efficiency of the reaction & t is time (in cycles) I can get this to work for most of my reactions, but part fails the nls fit (Convergence failure: iteration limit reached without convergence). This mainly has to do with the starting estimates for the nls function. I have used v...

Pete Biggs wrote: > >> There are devices that are using PXE-boot and require access to the company LAN. >> If I was to allow PXE-boot for unauthenticated devices, the whole thing would be >> pointless because it would defeat any security advantage that could be gained by >> requiring all devices and users to be authenticated: Anyone could bring a device >> capable

On 06/05/2015 03:43 PM, John R Pierce wrote: > On 6/5/2015 1:33 PM, Michael Hennebry wrote: >> It's a desktop in an old house. >> The outlets have ground-fault protection, >> but the third prong is ungrounded. > > not sure how GFI would function at all without a valid ground, unless > the GFI is wired to neutral, which is dangerous on its own. very. it defeats

> > Yes, I do it frequently with my phone. You do it once and it remembers > > it. My phone is more often on wifi than on 4G when I'm in a town. > > And you need to install certificates or enter a password or something? Yes. Just once, then things are remembered and you can seemlessly roam between various APs and networks. > > > > > Because you get faster

Dear Colleague, Happy New Year! As we know, codon preference among different species could be dramatically different. To enhance the expression level of a foreign protein in a particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very important to adjust the codon frequency of the foreign protein to match that of the host expression system. One classic example is GFP

Playing tf2, most of the time it runs very smoothly in fullscreen, and even in windowed, but occasionally, for a few frames, it either cuts to a distorted view of my virtual desktop (when I'm using one) or just random fluorescent colors set at right angles to each other. Anyone know what is happening? I can't take screens because, well, it's not taking them. Solutions appreciated!

Dear Colleague, Happy New Year! As we know, codon preference among different species could be dramatically different. To enhance the expression level of a foreign protein in a particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very important to adjust the codon frequency of the foreign protein to match that of the host expression system. One classic example is GFP

I am not sure if I should ask this question in this list. But I'll try. Currently I am trying to analyze images using EBImage and biOps. One of the features that I need to extract from various images is the color spectrum, namely, which colors each image consists of. So, each image hopefully can be converted into some sort of color histogram so that color ingredients are easily comparable

http://dovecot.org/test/ Hopefully better release than last one. Fixes several problems test66 had and also some older problems reported in this list. It also contains a pretty large mbox code cleanup, fixing several problems (especially related to X-IMAP/X-IMAPbase headers) and making it a bit faster. Maybe I'll backport it to 1.0-stable too when it looks like it's stable.. BTW. mbox

To add to the wish-list for "convolve": For modeling processes that decay exponentially in time, e.g., fluorescence, it is desirable to have a function that convolves an arbitrary vector with an exponential using an iterative method. In the TIMP package (which won't be on CRAN till R 2.5.0 is official, but is for now at www.nat.vu.nl/~kate/TIMP) we implemented this special-purpose function as "Conv1&qu...

On Fri, 5 Jun 2015, g wrote: > On 06/05/2015 03:43 PM, John R Pierce wrote: >> On 6/5/2015 1:33 PM, Michael Hennebry wrote: >>> It's a desktop in an old house. >>> The outlets have ground-fault protection, >>> but the third prong is ungrounded. >> >> not sure how GFI would function at all without a valid ground, unless >> the GFI is wired