search for: fluoresc

Hello R-help I have the following data for a standard curve concentration(nM),fluorescence 0,48.34 2,58.69 5,70.83 10,94.73 20,190.8 50,436.0 100, 957.9   (1)Is there function in R to plot a spline. (2)How can I interpolation,say 1000 point from 0nM-100nM and store this as a data frame of concentration,fluorescence (3)How can I modify the code below so that instead of retrieving a c...

Dear All, I am doing experiments in live plant tissue using a laser confocal microscope. The method is called "fluorescence recovery after photo-bleaching" (FRAP) and here follows a short summary: 1. Record/ measure fluorescence intensity in a defined, round region of interest (ROI, in this case a small spot) to determine the initial intensity value before the bleaching. This pre-bleach value is also used for...

...advance): I need to analisy a long list of data time \fluoscence I want to make one plot with standard deviation and the average data... Could you suggest me how to make that.. The data have this distribution: How can read and extrapolate this data? pippo.csv ps <- read.table(pippo.csv) time fluorescence 0; 0 4.013; 62.96 6.053 ;112.3 24.14; 106.2 ... How can use a plot with standard deviation of all data: time versus fluorescen

R-help, I have the following file I want to import to R (some lines removed) Calibrated CTD data for station:00280001 Calibrated:23/8 2001, Salinity Unsmoothed, Fluorescence Uncalibrated Maximum observed depth: 36 m QUAL has one digit for each of pressure, temp., sal. and fluor. QUAL=1:Uncal., QUAL=2:OK, QUAL=6:Interp., QUAL=9:No data DEPTH CTDPRS CTDTMP CTDSAL RAWFLU NUMB. QUAL M DBAR IPT...

...al, which is dangerous on its own. you might get a 3-prong-to-2-prong adapter and plug the PC into that, leaving the ground wire floating, and see if that works. As most desktops have the AC ground pin wired to the chassis, floating the chassis might well eliminate the hum. also, things like fluorescent lamps can greatly contribute to hum, although that often sounds like a buzz. lamp dimmers too, try switching any dimmers in the general area to either 100% on or totally off. > It's an Insignia NS-PAH5101 > It does not have a separate power source, > just plugs for the pink and...

Hi all, excuse me by this elementary question. I wish to know if a package in language R exists to analyze FCS (Fluorescence Correlation Spectroscopy) datas. And, if it possible, in addition can read the archives in raw format generated by the ConfoCor2 program. Thanks Horacio. **************************************************************************** Dr. Horacio Castellini http://ar.geocities.com/ho...

Hi all. I'd like know what is the format file saved by Leica Microsystems TCS SP2-AOBS equipped with a SP2-FCS2 Leica Microsystems workstation its datas. Cause it save in *.fcs extention file but ins't flow cytometry standart format file... Tahnks Horacio.

I haven't been able to fully make sense of the conflicting online information about whether and how to specify nesting structure for a nested, mixed model. I'll describe my experiment and hopefully somebody who knows lme4 well can help. We're measuring the fluorescence intensity of brain slices from frogs that have undergone various treatments. We want to use multcomp to look for differences between treatments, while accounting for the variance introduced by the random effects of brain and slice. There are a few measurements per slice, several slices per br...

Hi, Can anyone shed some light on this: My PC's are on a separate power circuit. Each PC has its own UPS. I have an old Honda generator. When the power goes out, I fire up the generator. However, the UPS's still run on battery. If I connect the PC directly to the socket and bypass the UPS, the PC works. I was told by a UPS supplier, that the power output from the generator was

Hello everyone, I have come across a bit of an odd problem: I am currently analysing data PCR reaction data of which part is behaving exponential. I would like to fit the exponential part to the following: y0 + alpha * E^t In which Y0 is the groundphase, alpha a fluorescence factor, E the efficiency of the reaction & t is time (in cycles) I can get this to work for most of my reactions, but part fails the nls fit (Convergence failure: iteration limit reached without convergence). This mainly has to do with the starting estimates for the nls function. I have us...

Pete Biggs wrote: > >> There are devices that are using PXE-boot and require access to the company LAN. >> If I was to allow PXE-boot for unauthenticated devices, the whole thing would be >> pointless because it would defeat any security advantage that could be gained by >> requiring all devices and users to be authenticated: Anyone could bring a device >> capable

...the PC into that, > leaving the ground wire floating, and see if that works. As most > desktops have the AC ground pin wired to the chassis, floating the > chassis might well eliminate the hum. no need. as he said "but the third prong is ungrounded". > also, things like fluorescent lamps can greatly contribute to hum, > although that often sounds like a buzz. lamp dimmers too, try > switching any dimmers in the general area to either 100% on or > totally off. correct. normally, repeat, normally, wiring for outlets and light are different wire runs, but they ma...

...ng it, you would get only 2Mbit. 4G isn't ubiquitous, 3G/EDGE is still common - and phone networks are patchy and slow. > > How do the shops prevent you from getting a phone signal? Why "prevent"? I never said that. Shops are essentially big metal boxes covered in wires and fluorescent lights, with the phone transmitter outside and an indeterminate distance away. Phone signals are weak and attenuated by the big metal box so your phone gives up on the network. Shops provide a "free" wifi as a service to its customers (nothing is free, they get the chance to harvest in...

...ly different. To enhance the expression level of a foreign protein in a particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very important to adjust the codon frequency of the foreign protein to match that of the host expression system. One classic example is GFP (green fluorescent protein) which was optimized to achieve high-level of expression in mammalian cells. GenScript has developed a proprietary algorithm for codon optimization. This algorithm can optimize sequences for protein expression using either your own codon usage table or those from publicly available co...

Playing tf2, most of the time it runs very smoothly in fullscreen, and even in windowed, but occasionally, for a few frames, it either cuts to a distorted view of my virtual desktop (when I'm using one) or just random fluorescent colors set at right angles to each other. Anyone know what is happening? I can't take screens because, well, it's not taking them. Solutions appreciated! Using ATI Radeon 3400 series, Catalyst 10.4 AMD Turion X2 Neo 64 1.6 ghz 4 gigs of ram.

...ly different. To enhance the expression level of a foreign protein in a particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very important to adjust the codon frequency of the foreign protein to match that of the host expression system. One classic example is GFP (green fluorescent protein) which was optimized to achieve high-level of expression in mammalian cells. GenScript has developed a proprietary algorithm for codon optimization. This algorithm can optimize sequences for protein expression using either your own codon usage table or those from publicly available co...

I am not sure if I should ask this question in this list. But I'll try. Currently I am trying to analyze images using EBImage and biOps. One of the features that I need to extract from various images is the color spectrum, namely, which colors each image consists of. So, each image hopefully can be converted into some sort of color histogram so that color ingredients are easily comparable

http://dovecot.org/test/ Hopefully better release than last one. Fixes several problems test66 had and also some older problems reported in this list. It also contains a pretty large mbox code cleanup, fixing several problems (especially related to X-IMAP/X-IMAPbase headers) and making it a bit faster. Maybe I'll backport it to 1.0-stable too when it looks like it's stable.. BTW. mbox

To add to the wish-list for "convolve": For modeling processes that decay exponentially in time, e.g., fluorescence, it is desirable to have a function that convolves an arbitrary vector with an exponential using an iterative method. In the TIMP package (which won't be on CRAN till R 2.5.0 is official, but is for now at www.nat.vu.nl/~kate/TIMP) we implemented this special-purpose function as "Conv...

...gt; not sure how GFI would function at all without a valid ground, unless >> the GFI is wired to neutral, which is dangerous on its own. As I understand it, GFI sums the currents going into each prong. If the sum is not close enough to zero, it breaks the circuit. >> also, things like fluorescent lamps can greatly contribute to hum, >> although that often sounds like a buzz. lamp dimmers too, try >> switching any dimmers in the general area to either 100% on or >> totally off. No dimmers, but I tried turning off the overhead flourescent lights. No effect. >> n...