Dear all, I would like to use the R's Bioconductor package flowCore to do flow cytometry analysis. I generated a FCS file using the file>export function of the FACSDiva Software Version 8 from a BD LSRII machine. I then used the functions: file.name <-system.file("extdata", "cd cells_FMO 8_003.fcs", package="flowCore") x <-read.FCS(file.name, transformation = FALSE) as shown in the flowCore: data structure package... vignette (20 May 2014) as available from the internet. However the result is an error: >Error in read.FCS(file.name, transformation = FALSE) : ' ' is not a valid file I then used the function: isFCSfile("cd cells_FMO 8_003.fcs") where cd cells_FMO 8_003.fcs is the name of the file. As expected I obtained the following message: >cd cells_FMO 8_003.fcs FALSE meaning I reckon that the file is not a FCS. Since I am completely new to this kind of analysis but I would not like to use flowJo, could anybody tell me how to load the FCS files? In the rest of the file I am pasting the beginning of the cd cells_FMO 8_003.fcs file for further reference (I can't attach the whole thing or even attaching the file because it is too big). From its gibberish I reckon that the encoding is probably wrong: I was expecting a flatfile after all not ASCII. Would the problem be how the run was exported? FlowJo however recognizes the files... Best regards, Luigi =============================FCS3.0 256 1927 1933 1192532 0 0 $BEGINANALYSIS0$ENDANALYSIS0$BEGINSTEXT0$ENDSTEXT0$BEGINDATA1933$ENDDATA1192532 $FIL180444.fcs$SYSWindows 7 6.1$TOT29765 $PAR10$MODEL$BYTEORD4,3,2,1$DATATYPEF$NEXTDATA0CREATORBD FACSDiva Software Version 8.0TUBE NAMEFMO 8$SRCcd cellsEXPERIMENT NAMEExperiment_001GUID4171c2f1-427b-4cc5-bf86-39bb76803c48$DATE31-OCT-2014$BTIM16:07:12$ETIM16:09:25SETTINGSCytometerWINDOW EXTENSION0.00EXPORT USER NAMELuigiMarongiuEXPORT TIME31-OCT-2014-16:07:11FSC ASF0.78AUTOBSTRUE$INST $TIMESTEP0.01SPILL3,405-450/50-A,405-655/8-A,405-525/50-A,1,0.0028442147740618787,0.0923076944711957,0,1,0,0.3425525014147933,0.08630456626553264,1APPLY COMPENSATIONTRUETHRESHOLDFSC,5000$P1NTime$P1R262144$P1B32$P1E0,0$P1G0.01P1BS0P1MS0$P2NFSC-A$P2R262144$P2B32$P2E0,0$P2V450$P2G1.0P2DISPLAYLINP2BS-1P2MS0$P3NFSC-H$P3R262144$P3B32$P3E0,0$P3V450$P3G1.0P3DISPLAYLINP3BS-1P3MS0$P4NFSC-W$P4R262144$P4B32$P4E0,0$P4V450$P4G1.0P4BS-1P4MS0$P5NSSC-A$P5R262144$P5B32$P5E0,0$P5V319$P5G1.0P5DISPLAYLINP5BS-1P5MS0$P6NSSC-H$P6R262144$P6B32$P6E0,0$P6V319$P6G1.0P6DISPLAYLINP6BS-1P6MS0$P7NSSC-W$P7R262144$P7B32$P7E0,0$P7V319$P7G1.0P7BS-1P7MS0$P8N405-450/50-A$P8Scd8 - pac blue$P8R262144$P8B32$P8E0,0$P8V450$P8G1.0P8DISPLAYLOGP8BS-1P8MS0$P9N405-655/8-A$P9Scd45ra - q655$P9R262144$P9B32$P9E0,0$P9V450$P9G1.0P9DISPLAYLOGP9BS-1P9MS0$P10N405-525/50-A$P10Sld - acqua$P10R262144$P10B32$P10E0,0$P10V450$P10G1.0P10DISPLAYLOGP10BS-1P10MS0CST BEADS EXPIREDFalse BHffE???GwI,E p F??gG? F{? D????G?C???BI33GA??G??GA G1?qG? ?G"? B?k?Ab=pB?.BI33E?-?G???E? Fe??G?h?Fc DN =?A??C??qBK33F??G?JF?? FV?G{?eF| Bp?Cb=pA? BM33G??G???G?? G???G?;G?? C??REY6?CiO\BO33E??fG?PlE?8 El G?4.E0 Cp??H?qC!??BQ33FK?G?U?F6 F?G??vF ?-?RC0? ?J BTffG^??G?m at G5L GH??G???G8? Ap?F??B???BV??F???G??dFr? G8??G???G!? C&?fB?? ??G?BZ F???G?)~F?b F???G???F ? ??=pB?W B \)B]33F?u?G?0?F?? G?.G?E?G ? B?=pF0fB=?HB_??E???G??dE?( FR?G???FE? Dg????C?z?Ba??F???G???F?? F??HG??:F?~ B?u?C#??BA??Bb??G?a?G?G?: G$?OG???G? C#??F??=B?ffBhffG??^G???Gw@ G~G?SF?( B?Q?F???CW BhffF? (G??OF?? F}v)G???FHL B????b=pB9? Bi??G?MG?i0G???GD?G?f?G*? B?\)C???CA??Bj G5[?G?s?GG G]?G??!G3 CR{F$HB?G?Bj F??G?/F? G(?G?G? B ffGxRC??Bl??E??G???E?? E??G??E? ??=pCtk?@?G?Bn??G???G?cG? G/$;G??]G!` B???F??BfG?BzffE??QG?:zE ? F0y G???F/? D?E???C???B{??G??\G?)JGz G,?G???G? B?k?G(??B?.B|ffG*<?G??}G? F???G??8F?` A?z???L?B?\B|??G?}\G??G???GY G???G@) C??qGp?CnB~??F??3G??pF? F??G??F?? C?G?F9 etc.
On 11/24/2014 06:18 AM, Luigi wrote:> Dear all, > I would like to use the R's Bioconductor package flowCore to do flow cytometryPlease address questions about Bioconductor packages to the Bioconductor support site https://support.bioconductor.org and...> analysis. > I generated a FCS file using the file>export function of the FACSDiva Software > Version 8 from a BD LSRII machine. I then used the functions: > file.name <-system.file("extdata", "cd cells_FMO 8_003.fcs", > package="flowCore")system.file() is used to access files installed in R packages, but probably you want to access your own file. Try file.name = file.choose() and selecting the file that you want to iniptu. Verify that the path is correct by displaying the result file.name Martin> x <-read.FCS(file.name, transformation = FALSE) > as shown in the flowCore: data structure package... vignette (20 May 2014) as > available from the internet. However the result is an error: > >Error in read.FCS(file.name, transformation = FALSE) : ' ' is not a valid > file > I then used the function: > isFCSfile("cd cells_FMO 8_003.fcs") > where cd cells_FMO 8_003.fcs is the name of the file. As expected I obtained the > following message: > >cd cells_FMO 8_003.fcs FALSE > meaning I reckon that the file is not a FCS. Since I am completely new to this > kind of analysis but I would not like to use flowJo, could anybody tell me how > to load the FCS files? In the rest of the file I am pasting the beginning of the > cd cells_FMO 8_003.fcs file for further reference (I can't attach the whole > thing or even attaching the file because it is too big). From its gibberish I > reckon that the encoding is probably wrong: I was expecting a flatfile after all > not ASCII. Would the problem be how the run was exported? FlowJo however > recognizes the files... > Best regards, > Luigi > > =============================> FCS3.0 256 1927 1933 1192532 0 0 > $BEGINANALYSIS0$ENDANALYSIS0$BEGINSTEXT0$ENDSTEXT0$BEGINDATA1933$ENDDATA1192532 > $FIL180444.fcs$SYSWindows 7 6.1$TOT29765 > $PAR10$MODEL$BYTEORD4,3,2,1$DATATYPEF$NEXTDATA0CREATORBD FACSDiva > Software Version 8.0TUBE NAMEFMO 8$SRCcd > cellsEXPERIMENT > NAMEExperiment_001GUID4171c2f1-427b-4cc5-bf86-39bb76803c48$DATE31-OCT-2014$BTIM16:07:12$ETIM16:09:25SETTINGSCytometerWINDOW > EXTENSION0.00EXPORT USER NAMELuigiMarongiuEXPORT > TIME31-OCT-2014-16:07:11FSC ASF0.78AUTOBSTRUE$INST > $TIMESTEP0.01SPILL3,405-450/50-A,405-655/8-A,405-525/50-A,1,0.0028442147740618787,0.0923076944711957,0,1,0,0.3425525014147933,0.08630456626553264,1APPLY > COMPENSATIONTRUETHRESHOLDFSC,5000$P1NTime$P1R262144$P1B32$P1E0,0$P1G0.01P1BS0P1MS0$P2NFSC-A$P2R262144$P2B32$P2E0,0$P2V450$P2G1.0P2DISPLAYLINP2BS-1P2MS0$P3NFSC-H$P3R262144$P3B32$P3E0,0$P3V450$P3G1.0P3DISPLAYLINP3BS-1P3MS0$P4NFSC-W$P4R262144$P4B32$P4E0,0$P4V450$P4G1.0P4BS-1P4MS0$P5NSSC-A$P5R262144$P5B32$P5E0,0$P5V319$P5G1.0P5DISPLAYLINP5BS-1P5MS0$P6NSSC-H$P6R262144$P6B32$P6E0,0$P6V319$P6G1.0P6DISPLAYLINP6BS-1P6MS0$P7NSSC-W$P7R262144$P7B32$P7E0,0$P7V319$P7G1.0P7BS-1P7MS0$P8N405-450/50-A$P8Scd8 > - pac > blue$P8R262144$P8B32$P8E0,0$P8V450$P8G1.0P8DISPLAYLOGP8BS-1P8MS0$P9N405-655/8-A$P9Scd45ra > - > q655$P9R262144$P9B32$P9E0,0$P9V450$P9G1.0P9DISPLAYLOGP9BS-1P9MS0$P10N405-525/50-A$P10Sld > - > acqua$P10R262144$P10B32$P10E0,0$P10V450$P10G1.0P10DISPLAYLOGP10BS-1P10MS0CST > BEADS EXPIREDFalse BHffE???GwI,E p F??gG? F{? > D????G?C???BI33GA??G??GA G1?qG? > ?G"? B?k?Ab=pB?.BI33E?-?G???E? Fe??G?h?Fc DN > =?A??C??qBK33F??G?JF?? FV?G{?eF| Bp?Cb=pA? BM33G??G???G?? G???G?;G?? > C??REY6?CiO\BO33E??fG?PlE?8 El G?4.E0 Cp??H?qC!??BQ33FK?G?U?F6 F?G??vF > ?-?RC0? ?J BTffG^??G?m at G5L GH??G???G8? Ap?F??B???BV??F???G??dFr? G8??G???G!? > C&?fB?? ??G?BZ F???G?)~F?b F???G???F ? ??=pB?W > B \)B]33F?u?G?0?F?? G?.G?E?G > ? B?=pF0fB=?HB_??E???G??dE?( FR?G???FE? Dg????C?z?Ba??F???G???F?? > F??HG??:F?~ B?u?C#??BA??Bb??G?a?G?G?: G$?OG???G? > C#??F??=B?ffBhffG??^G???Gw@ G~G?SF?( B?Q?F???CW > BhffF? > (G??OF?? F}v)G???FHL B????b=pB9? > Bi??G?MG?i0G???GD?G?f?G*? B?\)C???CA??Bj G5[?G?s?GG G]?G??!G3 > CR{F$HB?G?Bj F??G?/F? G(?G?G? B > ffGxRC??Bl??E??G???E?? E??G??E? ??=pCtk?@?G?Bn??G???G?cG? > G/$;G??]G!` B???F??BfG?BzffE??QG?:zE ? F0y G???F/? > D?E???C???B{??G??\G?)JGz G,?G???G? B?k?G(??B?.B|ffG*<?G??}G? > F???G??8F?` A?z???L?B?\B|??G?}\G??G???GY > G???G@) C??qGp?CnB~??F??3G??pF? F??G??F?? C?G?F9 > > etc. > > > > ______________________________________________ > R-help at r-project.org mailing list > https://stat.ethz.ch/mailman/listinfo/r-help > PLEASE do read the posting guide http://www.R-project.org/posting-guide.html > and provide commented, minimal, self-contained, reproducible code. >-- Computational Biology / Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109 Location: Arnold Building M1 B861 Phone: (206) 667-2793
Wrong list. See http://www.bioconductor.org/help/support/ --------------------------------------------------------------------------- Jeff Newmiller The ..... ..... Go Live... DCN:<jdnewmil at dcn.davis.ca.us> Basics: ##.#. ##.#. Live Go... Live: OO#.. Dead: OO#.. Playing Research Engineer (Solar/Batteries O.O#. #.O#. with /Software/Embedded Controllers) .OO#. .OO#. rocks...1k --------------------------------------------------------------------------- Sent from my phone. Please excuse my brevity. On November 24, 2014 6:18:07 AM PST, Luigi <marongiu.luigi at gmail.com> wrote:>Dear all, >I would like to use the R's Bioconductor package flowCore to do flow >cytometry analysis. >I generated a FCS file using the file>export function of the FACSDiva >Software Version 8 from a BD LSRII machine. I then used the functions: > file.name <-system.file("extdata", "cd cells_FMO 8_003.fcs", >package="flowCore") > x <-read.FCS(file.name, transformation = FALSE) >as shown in the flowCore: data structure package... vignette (20 May >2014) as available from the internet. However the result is an error: > >Error in read.FCS(file.name, transformation = FALSE) : ' ' is not >a valid file >I then used the function: > isFCSfile("cd cells_FMO 8_003.fcs") >where cd cells_FMO 8_003.fcs is the name of the file. As expected I >obtained the following message: > >cd cells_FMO 8_003.fcs FALSE >meaning I reckon that the file is not a FCS. Since I am completely new >to this kind of analysis but I would not like to use flowJo, could >anybody tell me how to load the FCS files? In the rest of the file I am > >pasting the beginning of the cd cells_FMO 8_003.fcs file for further >reference (I can't attach the whole thing or even attaching the file >because it is too big). From its gibberish I reckon that the encoding >is >probably wrong: I was expecting a flatfile after all not ASCII. Would >the problem be how the run was exported? FlowJo however recognizes the >files... >Best regards, >Luigi > >=============================>FCS3.0 256 1927 1933 1192532 >0 0 >>$BEGINANALYSIS>0>$ENDANALYSIS>0>$BEGINSTEXT>0>$ENDSTEXT>0>$BEGINDATA>1933>$ENDDATA>1192532 > >>$FIL>180444.fcs>$SYS>Windows 7 6.1>$TOT>29765 >>$PAR>10>$MODE>L>$BYTEORD>4,3,2,1>$DATATYPE>F>$NEXTDATA>0>CREATOR>BD >FACSDiva Software Version 8.0>TUBE >NAME>FMO 8>$SRC>cd cells>EXPERIMENT >NAME>Experiment_001>GUID>4171c2f1-427b-4cc5-bf86-39bb76803c48>$DATE>31-OCT-2014>$BTIM>16:07:12>$ETIM>16:09:25>SETTINGS>Cytometer>WINDOW > >EXTENSION>0.00>EXPORT USER NAME>LuigiMarongiu>EXPORT >TIME>31-OCT-2014-16:07:11>FSC ASF>0.78>AUTOBS>TRUE>$INST> >>$TIMESTEP>0.01>SPILL>3,405-450/50-A,405-655/8-A,405-525/50-A,1,0.0028442147740618787,0.0923076944711957,0,1,0,0.3425525014147933,0.08630456626553264,1>APPLY > >COMPENSATION>TRUE>THRESHOLD>FSC,5000>$P1N>Time>$P1R>262144>$P1B>32>$P1E>0,0>$P1G>0.01>P1BS>0>P1MS>0>$P2N>FSC-A>$P2R>262144>$P2B>32>$P2E>0,0>$P2V>450>$P2G>1.0>P2DISPLAY>LIN>P2BS>-1>P2MS>0>$P3N>FSC-H>$P3R>262144>$P3B>32>$P3E>0,0>$P3V>450>$P3G>1.0>P3DISPLAY>LIN>P3BS>-1>P3MS>0>$P4N>FSC-W>$P4R>262144>$P4B>32>$P4E>0,0>$P4V>450>$P4G>1.0>P4BS>-1>P4MS>0>$P5N>SSC-A>$P5R>262144>$P5B>32>$P5E>0,0>$P5V>319>$P5G>1.0>P5DISPLAY>LIN>P5BS>-1>P5MS>0>$P6N>SSC-H>$P6R>262144>$P6B>32>$P6E>0,0>$P6V>319>$P6G>1.0>P6DISPLAY>LIN>P6BS>-1>P6MS>0>$P7N>SSC-W>$P7R>262144>$P7B>32>$P7E>0,0>$P7V>319>$P7G>1.0>P7BS>-1>P7MS>0>$P8N>405-450/50-A>$P8S>cd8 > >- pac >blue>$P8R>262144>$P8B>32>$P8E>0,0>$P8V>450>$P8G>1.0>P8DISPLAY>LOG>P8BS>-1>P8MS>0>$P9N>405-655/8-A>$P9S>cd45ra > >- >q655>$P9R>262144>$P9B>32>$P9E>0,0>$P9V>450>$P9G>1.0>P9DISPLAY>LOG>P9BS>-1>P9MS>0>$P10N>405-525/50-A>$P10S>ld > >- >acqua>$P10R>262144>$P10B>32>$P10E>0,0>$P10V>450>$P10G>1.0>P10DISPLAY>LOG>P10BS>-1>P10MS>0>CST > >BEADS EXPIRED>False> BHffE???GwI,E p F??gG? F{? >D????G?C???BI33GA??G??GA G1?qG? >?G"? B?k?Ab=pB?.BI33E?-?G???E? Fe??G?h?Fc DN >=?A??C??qBK33F??G?JF?? FV?G{?eF| Bp?Cb=pA? BM33G??G???G?? >G???G?;G?? C??REY6?CiO\BO33E??fG?PlE?8 El G?4.E0 >Cp??H?qC!??BQ33FK?G?U?F6 F?G??vF ?-?RC0? ?J BTffG^??G?m at G5L >GH??G???G8? Ap?F??B???BV??F???G??dFr? G8??G???G!? C&?fB?? ??G?BZ >F???G?)~F?b F???G???F ? ??=pB?W >B \)B]33F?u?G?0?F?? G?.G?E?G >? B?=pF0fB=?HB_??E???G??dE?( FR?G???FE? Dg????C?z?Ba??F???G???F??>F??HG??:F?~ B?u?C#??BA??Bb??G?a?G?G?: G$?OG???G? >C#??F??=B?ffBhffG??^G???Gw@ G~G?SF?( B?Q?F???CW >BhffF? >(G??OF?? F}v)G???FHL B????b=pB9? >Bi??G?MG?i0G???GD?G?f?G*? B?\)C???CA??Bj G5[?G?s?GG >G]?G??!G3 CR{F$HB?G?Bj F??G?/F? G(?G?G>? B>ffGxRC??Bl??E??G???E?? E?>?G??E? ??=pCtk?@?G?Bn??G???G?c G?>G/$;G??]G!` B???F??BfG?BzffE??QG?:zE ? F0y G???F/?>D?E???C???B{??G??\G?)JGz G,?G???G? B?k?G(??B?.B|ffG*<?G??}G?>F???G??8F?` A?z???L?B?\B|??G?}\G??G???GY>G???G@) C??qGp?CnB~??F??3G??pF? F??G??F?? C?G?F9 > >etc. > > > >------------------------------------------------------------------------ > >______________________________________________ >R-help at r-project.org mailing list >https://stat.ethz.ch/mailman/listinfo/r-help >PLEASE do read the posting guide >http://www.R-project.org/posting-guide.html >and provide commented, minimal, self-contained, reproducible code.
If help files used the mustWork=TRUE argument to system.file() this sort of problem would become more apparent to the user. It would give a clear error message from system.file() instead of a mysterious error about file "" not being valid or, worse, a hang from an input command waiting for the user to type something into standard input (because scan() and others treat file="" the same as scan=stdin()). Bill Dunlap TIBCO Software wdunlap tibco.com On Mon, Nov 24, 2014 at 10:36 AM, Martin Morgan <mtmorgan at fredhutch.org> wrote:> On 11/24/2014 06:18 AM, Luigi wrote: > >> Dear all, >> I would like to use the R's Bioconductor package flowCore to do flow >> cytometry >> > > Please address questions about Bioconductor packages to the Bioconductor > support site > > https://support.bioconductor.org > > and... > > analysis. >> I generated a FCS file using the file>export function of the FACSDiva >> Software >> Version 8 from a BD LSRII machine. I then used the functions: >> file.name <-system.file("extdata", "cd cells_FMO 8_003.fcs", >> package="flowCore") >> > > system.file() is used to access files installed in R packages, but > probably you want to access your own file. Try > > file.name = file.choose() > > and selecting the file that you want to iniptu. Verify that the path is > correct by displaying the result > > file.name > > Martin > > x <-read.FCS(file.name, transformation = FALSE) >> as shown in the flowCore: data structure package... vignette (20 May >> 2014) as >> available from the internet. However the result is an error: >> >Error in read.FCS(file.name, transformation = FALSE) : ' ' is not >> a valid >> file >> I then used the function: >> isFCSfile("cd cells_FMO 8_003.fcs") >> where cd cells_FMO 8_003.fcs is the name of the file. As expected I >> obtained the >> following message: >> >cd cells_FMO 8_003.fcs FALSE >> meaning I reckon that the file is not a FCS. Since I am completely new to >> this >> kind of analysis but I would not like to use flowJo, could anybody tell >> me how >> to load the FCS files? In the rest of the file I am pasting the beginning >> of the >> cd cells_FMO 8_003.fcs file for further reference (I can't attach the >> whole >> thing or even attaching the file because it is too big). From its >> gibberish I >> reckon that the encoding is probably wrong: I was expecting a flatfile >> after all >> not ASCII. Would the problem be how the run was exported? FlowJo however >> recognizes the files... >> Best regards, >> Luigi >> >> =============================>> FCS3.0 256 1927 1933 1192532 0 0 >> $BEGINANALYSIS 0 $ENDANALYSIS 0 $BEGINSTEXT 0 $ENDSTEXT 0 $BEGINDATA 1933 >> $ENDDATA 1192532 >> $FIL 180444.fcs $SYS Windows 7 6.1 $TOT 29765 >> $PAR 10 $MODE L $BYTEORD 4,3,2,1 $DATATYPE F $NEXTDATA 0 CREATOR BD >> FACSDiva >> Software Version 8.0 TUBE NAME FMO 8 $SRC cd >> cells EXPERIMENT >> NAME Experiment_001 GUID 4171c2f1-427b-4cc5-bf86-39bb76803c48 $DATE >> 31-OCT-2014 $BTIM 16:07:12 $ETIM 16:09:25 SETTINGS Cytometer WINDOW >> EXTENSION 0.00 EXPORT USER NAME LuigiMarongiu EXPORT >> TIME 31-OCT-2014-16:07:11 FSC ASF 0.78 AUTOBS TRUE $INST >> $TIMESTEP 0.01 SPILL 3,405-450/50-A,405-655/8-A,405-525/50-A,1,0. >> 0028442147740618787,0.0923076944711957,0,1,0,0.3425525014147933,0.08630456626553264,1 >> APPLY >> COMPENSATION TRUE THRESHOLD FSC,5000 $P1N Time $P1R 262144 $P1B 32 $P1E >> 0,0 $P1G 0.01 P1BS 0 P1MS 0 $P2N FSC-A $P2R 262144 $P2B 32 $P2E 0,0 $P2V >> 450 $P2G 1.0 P2DISPLAY LIN P2BS -1 P2MS 0 $P3N FSC-H $P3R 262144 $P3B 32 >> $P3E 0,0 $P3V 450 $P3G 1.0 P3DISPLAY LIN P3BS -1 P3MS 0 $P4N FSC-W $P4R >> 262144 $P4B 32 $P4E 0,0 $P4V 450 $P4G 1.0 P4BS -1 P4MS 0 $P5N SSC-A $P5R >> 262144 $P5B 32 $P5E 0,0 $P5V 319 $P5G 1.0 P5DISPLAY LIN P5BS -1 P5MS 0 $P6N >> SSC-H $P6R 262144 $P6B 32 $P6E 0,0 $P6V 319 $P6G 1.0 P6DISPLAY LIN P6BS -1 >> P6MS 0 $P7N SSC-W $P7R 262144 $P7B 32 $P7E 0,0 $P7V 319 $P7G 1.0 P7BS -1 >> P7MS 0 $P8N 405-450/50-A $P8S cd8 >> - pac >> blue $P8R 262144 $P8B 32 $P8E 0,0 $P8V 450 $P8G 1.0 P8DISPLAY LOG P8BS -1 >> P8MS 0 $P9N 405-655/8-A $P9S cd45ra >> - >> q655 $P9R 262144 $P9B 32 $P9E 0,0 $P9V 450 $P9G 1.0 P9DISPLAY LOG P9BS -1 >> P9MS 0 $P10N 405-525/50-A $P10S ld >> - >> acqua $P10R 262144 $P10B 32 $P10E 0,0 $P10V 450 $P10G 1.0 P10DISPLAY LOG >> P10BS -1 P10MS 0 CST >> BEADS EXPIRED False BHffE???GwI,E p F ?gG? F{? >> D???? G?C???BI33GA??G? ?G A G1?qG? >> ?G" B?k?Ab=pB?. BI33E?-?G ??E? Fe ?G h?Fc DN >> =?A??C??qBK33F?? G?J F?? F V?G{?eF | B p?Cb=pA? BM33G? ?G???G?? G???G? >> ;G?? >> C??REY6 CiO\BO33E??fG?PlE?8 E l G?4.E 0 C p??H?qC!??BQ33FK? G U?F6 F ? >> G??vF ?-?RC0? ?J BTffG^??G m at G5L GH??G???G8? A p?F ? >> B???BV??F???G??dFr? G8??G???G!? >> C&?fB?? ? G?BZ F???G?)~F?b F???G ??F ? ??=pB?W >> B \)B]33F?u?G?0?F?? G ?.G?E?G >> ? B?=pF0 fB=?HB_??E???G??dE?( FR? G???FE? Dg??? ? C?z?Ba??F???G? ?F?? >> F??HG ?:F?~ B?u?C#??BA??Bb??G?a?G? G?: G$?OG???G ? >> C#??F??=B ffBhffG??^G???Gw@ G ~G SF?( B?Q?F???C W >> BhffF? >> (G??OF?? F}v)G???FHL B????b=pB9? >> Bi??G?M G?i0G???GD? G?f?G*? B?\)C???CA??Bj G5[?G?s?G G G ]?G??!G 3 >> CR {F $HB G?Bj F?? G?/ F? G (?G? G ? B >> ffG xRC ??Bl??E? ?G?? E?? E? ?G?? E? ??=pCtk?@ G?Bn??G???G?c G? >> G/$;G??]G!` B???F? ?BfG?BzffE??QG :zE ? F0y G???F/? >> D?E ??? C???B{??G??\G?)JGz G,? G???G ? B k?G(??B?. B|ffG*<?G??}G ? >> F???G ?8F?` A?z???L?B \B|??G?}\G?? G ??GY >> G ??G@) C??qG p?C n B~??F??3G??pF? F? ?G? ?F?? C G?F9 >> >> etc. >> >> >> >> ______________________________________________ >> R-help at r-project.org mailing list >> https://stat.ethz.ch/mailman/listinfo/r-help >> PLEASE do read the posting guide http://www.R-project.org/ >> posting-guide.html >> and provide commented, minimal, self-contained, reproducible code. >> >> > > -- > Computational Biology / Fred Hutchinson Cancer Research Center > 1100 Fairview Ave. N. > PO Box 19024 Seattle, WA 98109 > > Location: Arnold Building M1 B861 > Phone: (206) 667-2793 > > > ______________________________________________ > R-help at r-project.org mailing list > https://stat.ethz.ch/mailman/listinfo/r-help > PLEASE do read the posting guide > http://www.R-project.org/posting-guide.html > and provide commented, minimal, self-contained, reproducible code. > >[[alternative HTML version deleted]]