Hi, I have a problem with doing a repeated measures ANOVA. I will first give you an idea of what my dataset looks like. We have 20 ponds, and for each pond we took some individuals (waterfleas), say 10 and tested them along two treatments (A and B). Now, for each pond we also know the fish background (Fish versus No Fish) and Land use intensity (High versus Low). Now, we measure the offspring of the first clutch and second clutch of each individual, and we want to see if there is an effect of each of the other factors (treatment, background, land use), so we need to do a repeated measurement. So I rearranged my data where I put the first and second clutch in one column, something like this: Ponds Treatment Background LandUse Clutch SizeClutch Pond1 A Fish High 1 10 Pond1 B Fish High 1 15 Pond2 A NoFish High 1 8 Pond2 B NoFish High 1 11 etc Pond20 B Fish Low 1 12 Pond1 A Fish High 2 15 Pond1 B Fish High 2 17 Pond2 A NoFish High 2 16 Pond2 B NoFish High 2 18 etc Pond20 B Fish Low 2 11 So I made the Clutch variable myself, to separate between the different clutches. I then performered the following analysis summary(aov(SizeClutch ~ Treatment*Background*LandUse + Error(Clutch/Treatment*Background*LandUse) + Ponds , random = ~ Ponds| Background*LandUse/Ponds)) This gives me something, but not exactly what I thought I would get. I want to have results for Ponds LandUse Background Treatment LandUse*Background LandUse*Treatment LandUse*Background*Treatment Clutch Clutch*Ponds Clutch*LandUse Clutch*Background Clutch*Treatment Clutch*LandUse*Background Clutch*LandUse*Treatment Clutch*LandUse*Background*Treatment and p values for all of the effects. Now, I don't know if it is possible to come to these results and if I'm doing the repeated measure ANOVA correctly. Also, are there other ways to do a repeated measurements in R? Thanks in advance. Lynn [[alternative HTML version deleted]]