I have been trying to run a Fisher's LSD for quite some time now, however the output I am receiving only ranks the means of each treatment in terms of significant difference, and will not give me the actual value of "Least Significant Difference" I am looking for. I am using a data set that has four replications and two factors ("Ratio" and "Species"), however for this particular dataset "Species" was not a significant factor so I am only interested in the LSD value for comparing the "Ratio" factor. Note that the response variable for two of the ratios only has three values (the fourth rep had to be deleted due to impossibility), and I do not know if this may be the source of my problem. Regardless, here is my script: f <- file.choose() svs30dayshoot <- read.csv(f) svs30dayshoot str(svs30dayshoot) svs30dayshoot$Ratio<-factor(svs30dayshoot$Ratio) aov30dayshoot <- aov(RY ~ Species*Ratio, data=svs30dayshoot) summary(aov30dayshoot) install.packages('agricolae') library(agricolae) LSD<-LSD.test(aov30dayshoot,"Ratio", p.adj="bonferroni") LSD And the resulting output is below. Note there is no "Least Significant Difference" value given. In fact, this output looks nothing like the output I'm used to seeing when running Fisher's LSD with a one-way ANOVA (in my class): $statistics Mean CV MSerror 0.6804658 107.7707 0.5377916 $parameters Df ntr bonferroni 28 5 3.046929 $means RY std.err r LCL UCL Min. Max. 0 0.0000000 0.2592758 8 -0.5311024 0.5311024 0.0000 0.0000 100 1.0000000 0.2592758 8 0.4688976 1.5311024 1.0000 1.0000 25 0.3140571 0.2771775 7 -0.2537152 0.8818295 0.0200 0.5069 50 0.8016875 0.2592758 8 0.2705851 1.3327899 0.3800 1.5952 75 1.3208286 0.2771775 7 0.7530562 1.8886010 0.3864 4.8889 $comparison NULL $groups trt means M 1 75 1.3208286 a 2 100 1.0000000 ab 3 50 0.8016875 ab 4 25 0.3140571 ab 5 0 0.0000000 b Any help would be greatly appreciated! -- View this message in context: http://r.789695.n4.nabble.com/Fisher-s-LSD-problem-tp4682153.html Sent from the R help mailing list archive at Nabble.com.