Hello, I would like to perform an NMDS on the following: I have two independent variables, which are sites and treatments. I have 6 sites which are peatlands. I collected 5 replicates (at the same time) from each of the sites. I used each of the replicates in a treatment. There were 4 treatments. 2 were controls and the other two were a simulated 30 day drought and a simulated 60 day drought.>From each of the replicates in each of the treatments I measured pH and nitrate, sulphate, dissolved organic carbon, calcium, magnesium, nickel, cobalt, aluminum, and copper concentrations.I tried performing an NMDS using the following tutorial http://strata.uga.edu/software/pdf/mdsTutorial.pdf. However, I did not take into account sites or treatments. All the data was used without making those distinctions. I would like to make an NMDS plot which distinguishes the points as sites and another plot which distinguishes the points as treatments but which still demonstrates where the pH, nitrate, sulphate, etc variables are located across the plot. Do you know how I can accomplish this? I hope I am clear in my explanation. Thank you, Myra [[alternative HTML version deleted]]