Hi all, I'm trying to perform a nested ANOVA, in which "Rewetted" is the main factor and "Position" is a fixed factor nested in "Location". I'm interested in the effect of "Rewetting" and "Position" on the dependent variable (in this case called N), and also in the interaction between these factors. I tried finding this using this script: summary(aov(data=data1, N~Rewetted*Location+Error(Position/Location)) This yielded the following output: Error: Position Df Sum Sq Mean Sq F value Pr(>F) Residuals 2 0.1625 0.08125 Error: Position:Location Df Sum Sq Mean Sq F value Pr(>F) Rewetted 1 29.65 29.650 5.664 0.0321 * Location 6 42.61 7.102 1.357 0.2975 Residuals 14 73.29 5.235 --- Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1 Error: Within Df Sum Sq Mean Sq F value Pr(>F) Residuals 48 109.9 2.289 As you can see, there is no P-value for Error: position, so I can't say what the effect of Position is. According to my supervisor this is because something in the formulation of the script is wrong, but he won't tell me what (or he doesn't actually know...). What should I change? Thanks, Annette [[alternative HTML version deleted]]