Greetings I went back over the examples in the R Graphics book on the grid webpage and saw an example which I have adapted and seems to work: pushViewport(viewport(layout=grid.layout(nrow=26,ncol=2))) pushViewport(viewport(layout.pos.col=1,layout.pos.row=1)) plot_gene_map(dna_segs=list(segs[[1]][["seg"]]),annotations=segs[[1]] [["annot"]],dna_seg_scale=T, scale_cex = 1.4 ,dna_seg_line =F,plot_new=FALSE,scale=F,arrow_head_length=1,annotation_cex=1.4, main="A",main_pos="left") popViewport() pushViewport(viewport(layout.pos.col=1,layout.pos.row=2)) plot_gene_map(dna_segs=list(segs[[2]][["seg"]]),annotations=segs[[2]] [["annot"]],dna_seg_scale=T, scale_cex = 1.4 ,dna_seg_line =F,plot_new=FALSE,scale=F,arrow_head_length=1,annotation_cex=1.4, main="B",main_pos="left") popViewport() pushViewport(viewport(layout.pos.col=1,layout.pos.row=3)) plot_gene_map(dna_segs=list(segs[[3]][["seg"]]),annotations=segs[[3]] [["annot"]],dna_seg_scale=T, scale_cex = 1.4 ,dna_seg_line =F,plot_new=FALSE,scale=F,arrow_head_length=1,annotation_cex=1.4, main="C",main_pos="left") popViewport() There must be some error in the way I was calling the function plotpush in the previous email. Cheers Mike