Hi, I am looking at the change in N concentration in plant roots over 4 time points and I have fit a spline to the data using ns and lme: fit10 <- lme( N~ns(day, 3), data = rcn10G) I may want to adjust the model a little bit, but for now, let's assume it's good. I get output for the fixed effects: Fixed: N ~ ns(day, 3) (Intercept) ns(day, 3)1 ns(day, 3)2 ns(day, 3)3 1.15676524 0.14509171 0.04459627 0.09334428 and coefficients for each experimental unit in my experiment: (Intercept) ns(day, 3)1 ns(day, 3)2 ns(day, 3)3 24 1.050360 -0.42666159 -0.56290877 -0.10714407 13 1.104464 -0.30825350 -0.53311653 -0.05558150 31 1.147878 -0.14548512 -0.78673906 -0.07231781 46 1.177781 -0.22278380 -0.80278177 -0.02321460 15 1.144215 -0.04484519 -0.06084798 0.07633663 32 1.213007 0.00741061 0.03896933 0.15325849 23 1.274615 0.16477514 0.00872224 0.23128320 41 1.215626 0.57050767 0.11415467 0.10608867 43 1.134203 0.48070741 0.72112899 0.18108193 12 1.091422 0.39563632 1.01521528 0.22597459 21 1.100631 0.44589314 0.98526322 0.23535739 35 1.226980 0.82419937 0.39809568 0.16900841 NOW, I want to write a spline function where I can incorporate these coefficients to get the predicted N concentration value for each day. However, I am having trouble finding the right spline equation, since there are many forms on the internets. I know it won't be a simple one, but can some one direct me to the equation that would be best to use for ns? Thanks a lot, Ranae -- View this message in context: http://r.789695.n4.nabble.com/splines-and-ns-equation-tp4631986.html Sent from the R help mailing list archive at Nabble.com.