Hello! I have a binary data from DGGE gel with 20 samples. These samples are divided into 4 groups, so there are 4(groups) x 5(replicas) = 20. I used ANOSIM method, and everything is ok but ... I have a problem with boxplot... like this: ________________ distance <- vegdist(dgge2, method="jaccard") anosim(distance, var$Treatment) anosim <- anosim(distance, var$Treatment) plot(anosim, main = "ANOSIM test", xlab="Treatment", ylab="Distance") http://r.789695.n4.nabble.com/file/n4092261/anosim.jpeg Warning message: In bxp(list(stats = c(5.5, 56, 109, 141.25, 171, 5.5, 24, 24, 78.5, : some notches went outside hinges ('box'): maybe set notch=FALSE ___________________ My question is, it is possible to straighten this graph? If You have some better ideas how to compare these 4 groups basing on similarities, please tell me! -- View this message in context: http://r.789695.n4.nabble.com/ANOSIM-boxplot-problem-tp4092261p4092261.html Sent from the R help mailing list archive at Nabble.com.