Hi 1Rnwb,
I am a technical support for Hitachi Solutions and I saw your post regarding
ELISA analysis. When I opened up your .csv file, I noticed it was a Luminex
file from the Data Collector 1.7 software. My company developed a multiplex
data analysis software for the Luminex platform called
http://www.miraibio.com/masterplex-qt/qt-luminex-quantitative-data-analysis-software.html
MasterPlex QT . I used this software and did ran the curve-fitting for you
and included both the report (.csv file) with the final results and the
MasterPlex QT project file (.mlxq). Here is one of your standard curves for
"ENA-78(37)":
http://r.789695.n4.nabble.com/file/n3325361/StandardChart.png
http://r.789695.n4.nabble.com/file/n3325361/ds2_cs_panela_p2_101806.csv
ds2_cs_panela_p2_101806.csv
http://r.789695.n4.nabble.com/file/n3325361/ds2_cs_panela_p2_101806.mlxq
ds2_cs_panela_p2_101806.mlxq
I also noticed that quite a few of your samples had pretty low bead counts
(<35) where the data is not significant so they are outlined in the project
file. I would normally flag these as outliers but I just left all the data
intact. You'll be able to find this information and much more in my blog
post titled
http://www.miraibio.com/blog/2009/02/tips-for-luminex-data-analysis/ 10 Tips
for Luminex/Bio-Plex Data Analysis .
I hope this information helps. Feel free to contact me directly at aliu at
miraibio dot com if you have any questions regarding the Luminex data
analysis.
Allen Liu
--
View this message in context:
http://r.789695.n4.nabble.com/creating-standard-curves-for-ELISA-analysis-tp875877p3325361.html
Sent from the R help mailing list archive at Nabble.com.
