Dear, Thank you for cheking the mail! I'm Bo, student in TTU, and had a problem in using r for analyzing microarry data. This is what I write in r: library(limma) argets <- readTargets(file="targets.txt") *targets.txt is a tab-delimited file with columns: "SlideNumber", "FileName", "Cy3", "Cy5". The filenames refer to the feature extraction files for each array in the experiment. RG<-read.maimages(targets, source="agilent",wt.fun=myfunFE,columns=list(R="rProcessedSignal",G="gProcessedSignal")) *In this step , cannot open file'Imm 10 Cy3_TM 10 Cy5 ': No such file or directory ,and I 've tried to path= "d:\\Imm 10 Cy3_TM 10 Cy5" or setwd ,but the same error, so I donot know how to tell to find this file,could you help me to figure it out? Thank you so much! Sincerely, Bo