Hello all - I have been teaching myself R and have run into a problem that i cant seem to find an answer too. I appreciate any advice that can be given. My Design is a 2 level nested design with Site nested in the interaction of Region * Habitat The response variable i have been using are my quadrat counts The explanatory variables are below Region (a=2 levels - fixed) Habitat (b=2 levels - fixed) Site (c=16 sites - random) n=20 quadrats at each site My ANOVA table will look like the following df Region 1 (a-1) Habitat 1 (b-1) Region*Habitat 1 (a-1)(b-1) Site(Region*Habitat) 60 ab(c-1) Residual 1216 abc(n-1) The code i have been using in R looks like this Model1 <- lm(Count ~ Region + Habitat + Region*Habitat +Site%in%(Region*Habitat)) i have also tried Model1 <- lm(Count ~ Region + Habitat + Region*Habitat +Site%in%Region*Habitat) However i can not seem to get the correct degrees of freedom. Could someone please help me with the correct code to nest a factor within an interaction term. Thank you very much Stephen Cole Marine Ecology Lab Saint Francis Xavier University