Dear R-help! I ask you to help me with my problems with using R. First, I ask you to forgive my bad English! I try to use R in my study. Subject of my work is comparative study of flora of lakes in different regions of Russia. I have done floristical descriptions of 152 lakes (I think it's enough) and have tabulated it. As data I have a table, such has 152 rows (lakes) 290 variables (species of plants). Thus, there is frequency of species of plants in every cell of this table. I wanted to search some groups between these lakes. I have done this searching with cluster analysis (cutree(hclust(dist(DATA,"manh"),"ward"),4)). Then I apply principal component analysis: =============##loading data (DATA) ##loading the list of groups (GROUPS) d.prc<-princomp(DATA) palette(rainbow(length(unique(GROUPS)))) plot(d.prc$scores,type="n",main="Principal Component Analysis",xlab="Different groups of lakes have different color-labeling") text(d.prc$scores,labels=GROUPS,col=GROUPS,cex=.6) =============I have received attached plot as a result. Both I and my supervisor of studies are agree with clustering. You can see 4 groups in this figure. Write to me please your opinion, if these groups are authentic. Could you present any criteria of existence of distinguishable groups and any criteria of allocation of these groups? Can I use PCA as instrument to test visually clustering? I heard some opinion: "PCA is for visual allocating of the groups and then you can search hypothetical parameter to explain groupping. But you cannot use PCA for looking for the loadings (influence) of variables on the groupping". Is it true? Could you advise to me some other methods to analyze. ----------------------------------- Best wishes, Altshuler Eugenij P. mailto:lapotcarex at mtu-net.ru