Hello, I have a data set with 15 variables (first one is the response) and 1200 observations. Now I use pls package to do the plsr with cross validation as below. trainSet = as.data.frame(scale(trainSet, center = T, scale = T)) trainSet.plsr = mvr(formula, ncomp = 14, data = trainSet, method = "kernelpls", CV = TRUE, validation = "LOO", model = TRUE, x = TRUE, y = TRUE) after that I wish to obtain the value of "se", estimated standard errors of the estimates(cross validation), mentioned in the function of MSEP, but not implemented yet, so I made the program by myself to calculate it. The results I got seem not right, and I wonder which step below is wrong. y = trainSet.plsr$y p = as.data.frame(trainSet.plsr$validation$pred) i = 1; msep_element = matrix(nrow = 1200, ncol = 14) while(i <= length(p)){ msep_element[,i] = (p[i]-y)^2 i = i + 1 } msep = colMeans(msep_element) msep_sd = sd(msep_element) Then I compare "msep" with "trainSet.plsr$validation$MSEP", which are the same, but the values of "msep_sd" seem much larger than I expected, is it the same as "se"? If not, how to calculate "se" of cross validation? Thank you, Shengzhe