Hello sir:
As to the "variance stabilization" method which is applied in the
"vsn"package under R environment,here's a question about the ratio
of the 2 channels:
After applying vsn,we can get new_cy5 and new_cy3 intensity according to the
original cy5 and cy3 intensity respectively. And the new_cy5 new_cy3 are similar
with ln(intensity).
But how can I calculate the ratio=cy5/cy3 ?
If the new_cy5 and new_cy3 is log2(intensity),the ratio equals to
2^(log2cy5-log2cy3),but as to vsn,how to get the ratio?
Since the vsn transformation is similar to ln transformation when the intensity
is high, I wanna know how high the intensity is so that I can use ln instead of
vsn?
Thanks a lot!
my best regards!
