Waverley @ Palo Alto
2014-Apr-04 20:27 UTC
[R] solicit help to read in 384 plate color image
Hi, I am doing an experiment which results different colors of different intensities in the 384 micro titer plate. I took a picture of the plate by scanning in the image as a jpeg file and now I want to 1. read in the image file 2. grid the content 3. need to extract the intensity and color of each well. Is there a R package I can use for that? there is a package called "gitter" which is almost satisfying my needs. However, it can only read in grey values and no colors. If someone has the code, please share. -- Thanks. waverley [[alternative HTML version deleted]]
Hi There was package rimage which could read jpeg file in colours and manipulate with it. However it is not actively maintained and therefore it does not work in recent versions of R. I believe that R 2.14.0 is the last version able to use rimage. It was quite simple and I was tempted to start its maintenance but I do not use it often and so my interest ceased. If you wanted to try it you need to install this old version, install rimage. After that you can read your images in this old version, preprocess it with rimage and save result for use in new R version. Regards Petr PS. If you do not find rimage in CRAN I can send you a zip file.> -----Original Message----- > From: r-help-bounces at r-project.org [mailto:r-help-bounces at r- > project.org] On Behalf Of Waverley @ Palo Alto > Sent: Friday, April 04, 2014 10:28 PM > To: r-help > Subject: [R] solicit help to read in 384 plate color image > > Hi, > > I am doing an experiment which results different colors of different > intensities in the 384 micro titer plate. > > I took a picture of the plate by scanning in the image as a jpeg file > and now I want to > > 1. read in the image file > 2. grid the content > 3. need to extract the intensity and color of each well. > > Is there a R package I can use for that? there is a package called > "gitter" > which is almost satisfying my needs. However, it can only read in grey > values and no colors. > > If someone has the code, please share. > > -- > > Thanks. > > waverley > > [[alternative HTML version deleted]] > > ______________________________________________ > R-help at r-project.org mailing list > https://stat.ethz.ch/mailman/listinfo/r-help > PLEASE do read the posting guide http://www.R-project.org/posting- > guide.html > and provide commented, minimal, self-contained, reproducible code.________________________________ Tento e-mail a jak?koliv k n?mu p?ipojen? dokumenty jsou d?v?rn? a jsou ur?eny pouze jeho adres?t?m. Jestli?e jste obdr?el(a) tento e-mail omylem, informujte laskav? neprodlen? jeho odes?latele. Obsah tohoto emailu i s p??lohami a jeho kopie vyma?te ze sv?ho syst?mu. Nejste-li zam??len?m adres?tem tohoto emailu, nejste opr?vn?ni tento email jakkoliv u??vat, roz?i?ovat, kop?rovat ?i zve?ej?ovat. Odes?latel e-mailu neodpov?d? za eventu?ln? ?kodu zp?sobenou modifikacemi ?i zpo?d?n?m p?enosu e-mailu. V p??pad?, ?e je tento e-mail sou??st? obchodn?ho jedn?n?: - vyhrazuje si odes?latel pr?vo ukon?it kdykoliv jedn?n? o uzav?en? smlouvy, a to z jak?hokoliv d?vodu i bez uveden? d?vodu. - a obsahuje-li nab?dku, je adres?t opr?vn?n nab?dku bezodkladn? p?ijmout; Odes?latel tohoto e-mailu (nab?dky) vylu?uje p?ijet? nab?dky ze strany p??jemce s dodatkem ?i odchylkou. - trv? odes?latel na tom, ?e p??slu?n? smlouva je uzav?ena teprve v?slovn?m dosa?en?m shody na v?ech jej?ch n?le?itostech. - odes?latel tohoto emailu informuje, ?e nen? opr?vn?n uzav?rat za spole?nost ??dn? smlouvy s v?jimkou p??pad?, kdy k tomu byl p?semn? zmocn?n nebo p?semn? pov??en a takov? pov??en? nebo pln? moc byly adres?tovi tohoto emailu p??padn? osob?, kterou adres?t zastupuje, p?edlo?eny nebo jejich existence je adres?tovi ?i osob? j?m zastoupen? zn?m?. This e-mail and any documents attached to it may be confidential and are intended only for its intended recipients. If you received this e-mail by mistake, please immediately inform its sender. Delete the contents of this e-mail with all attachments and its copies from your system. If you are not the intended recipient of this e-mail, you are not authorized to use, disseminate, copy or disclose this e-mail in any manner. The sender of this e-mail shall not be liable for any possible damage caused by modifications of the e-mail or by delay with transfer of the email. In case that this e-mail forms part of business dealings: - the sender reserves the right to end negotiations about entering into a contract in any time, for any reason, and without stating any reasoning. - if the e-mail contains an offer, the recipient is entitled to immediately accept such offer; The sender of this e-mail (offer) excludes any acceptance of the offer on the part of the recipient containing any amendment or variation. - the sender insists on that the respective contract is concluded only upon an express mutual agreement on all its aspects. - the sender of this e-mail informs that he/she is not authorized to enter into any contracts on behalf of the company except for cases in which he/she is expressly authorized to do so in writing, and such authorization or power of attorney is submitted to the recipient or the person represented by the recipient, or the existence of such authorization is known to the recipient of the person represented by the recipient.