Hi,
This is a quiet a specific question, but i really need help.
I'm using the flowCore package coming from BioConductor.
No i would like to do a transformation, a log tranformation.
Although i think i put everything like it should, i get this error:
Error in log(x, logbase) : Non-numeric argument to mathematical function
which looks like a general calculating problem.
the code i use now is the following:
logTransi <- truncateTransform("truncate at 1", a=1)
testSetLog <- transform(testSet, 'FL10'= logTransi('FL10'),
'FL11'=logTransi('FL11'))
testSet looks like this:
> testSet
flowFrame object 'MarjanD.20120926.A.0026.fcs'
with 100000 cells and 14 observables:
name desc range minRange maxRange
$P1 pulse.width <NA> 65536 768 65535
$P2 FSC_PER <NA> 65536 0 65535
$P3 FSC_PAR <NA> 65536 0 65535
$P4 SSC <NA> 65536 0 65535
$P5 FL01 <NA> 65536 0 65535
$P6 FL02 <NA> 65536 0 65535
$P7 FL03 <NA> 65536 0 65535
$P8 FL04 <NA> 65536 0 65535
$P9 FL05 <NA> 65536 0 65535
$P10 FL10 <NA> 65536 0 65535
$P11 FL11 <NA> 65536 0 65535
$P12 FL12 <NA> 65536 0 65535
$P13 FL13 <NA> 65536 0 65535
$P14 TIME <NA> 65536 47185 65535
78 keywords are stored in the 'description' slot
Other transformation (truncateTransform) don't give an error, this one
does... Any help?
kind regards,
Pieter
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