Displaying 20 results from an estimated 1000 matches similar to: "creating standard curves for ELISA analysis"
2008 Dec 26
1
starting values update
Hi all,
does anyone know how to automatically update starting values in R?
I' m fitting multiple nonlinear models and would like to know how I can update starting values without having to type them in.
thank all
--- On Fri, 12/26/08, r-help-request@r-project.org <r-help-request@r-project.org> wrote:
From: r-help-request@r-project.org <r-help-request@r-project.org>
Subject:
2013 Jan 03
2
Sas by function in R
Hello,
It's an alternative to use SAS by function in R?
I want to plot d histograms by plot.from example bellow:
Thank you!
plot d
1 1 16.3
2 1 25.0
3 1 57.8
4 1 17.0
5 2 10.8
13 2 96.4
17 3 76.0
18 3 32.0
19 3 11.0
20 3 11.0
24 3 106.0
25 3 12.5
21 4 19.3
22 4 12.0
26 4 15.0
27 5 99.3
32 7 11.0
36
2011 Feb 01
4
Fitting ELISA measurements "unknowns" to 4 parameter logistic model
Hello,
I am trying to fit my Elisa results (absorbance readings) to a standard
curve. To create the standard curve model, I performed a 4-parameter
logistic fit using the 'drc' package (ExpectedConc~Absorbance). This gave me
the following:
> FourP
A 'drc' model.
Call:
drm(formula = Response ~ Expected, data = SC, fct = LL.4())
Coefficients:
b:(Intercept) c:(Intercept)
2012 Nov 14
2
aggrete data from combination
Dear R users,
A have a dataframe (matrix) with two collumns (plot, and diameter (d)). I
want all diameters values for different combination of plots.
For example I want all d values for all posible combination, 100C2 (all d
values for plot 1 with all d values in the plot 2.......with all d values
from plot 1 with all d values from plot 100, ...... with all d values from
plot 99 with all d values
2011 Feb 25
0
creating standard curves for ELISA analysis
Hi 1Rnwb,
I am a technical support for Hitachi Solutions and I saw your post regarding
ELISA analysis. When I opened up your .csv file, I noticed it was a Luminex
file from the Data Collector 1.7 software. My company developed a multiplex
data analysis software for the Luminex platform called
http://www.miraibio.com/masterplex-qt/qt-luminex-quantitative-data-analysis-software.html
MasterPlex
2013 Jan 03
5
splitting matrices
Dear useRs,
i want to split a matrix having 1116rows and 12 columns. i want to split that matrix into 36 small matrices each having 12 columns and 31 rows. The big matrix should be splitted row wise. which means that the first small matrix should copy values which are in first 31 rows and 12 columns of the big matrix. similarly 2nd small matrix should contain values from 32nd to 63rd row of the
2013 Jun 12
1
Question on Simple Repeated Loops
Dear R-User,
Appreciate any helps. It looks simple, but I don't have a clue.
Given that I have a dataframe of tree population with three variables:
sp=species ,
d0=initial_size
grow=growth increment from initial size per year
How can I calculate the future growth increment of each tree for the next 3 years.
The following Rscript was written,
#----------
a0 <-
2007 Aug 09
2
Systematically biased count data regression model
Dear all,
I am attempting to explain patterns of arthropod family richness
(count data) using a regression model. It seems to be able to do a
pretty good job as an explanatory model (i.e. demonstrating
relationships between dependent and independent variables), but it has
systematic problems as a predictive model: It is biased high at low
observed values of family richness and biased low at
2012 Nov 14
5
aggregate combination data
Dear R users,
I want to aggregate all *d *data from all combination of n *plots* taken
by k.
Thank very much!
My data is like that:
plot d 1 14 1 13 1 12 1 14 1 18 1 20 1 21 1
43 1 108 1 43 2 41 2 61 2 83 2 61 2 84 2 45 2 21 2 12 2 11 ... 100
10
100 12
--
---
Catalin-Constantin ROIBU
Forestry engineer, PhD
Forestry Faculty of Suceava
2009 Jul 29
3
Side by Side Barplot Newbie Question
Hi,
Many apologies for sending this twice. I accidentally hit the send button
before I finished writing my mail. I am new to R and I hope someone can
help me with my problem. I am trying to draw a side by side barplot.
There is a main experiment and there are many sub experiments within the
main experiment. I would like to draw a bar plot showing the number and type
of sub_experiments done for
2009 Aug 13
3
shading between two smoothed curves
I have a set of 52 weekly values, one is the desired high lake stage for
a week, and the other is the desired low lake stage for each week. It
looks like this:
week
High
Low
1
16
14.5
2
16
14.5
3
15.95
14.45
4
15.84
14.34
5
15.73
14.23
6
15.61
14.11
7
15.5
14
8
15.38
13.88
9
15.25
13.75
10
15.13
13.63
....
52
15
13.5
I can plot out two nice curves
2003 Jul 11
2
troubleshooting help
Hi,
I've installed Samba and started up the processes; however, I do not see the
Samba server in Network Neighborhood. In using the DIAGNOSIS.txt file, I
cannot get past test 3: ./smbclient -U% -L localhost.
The output is as follows:
added interface ip=140.188.120.55 bcast=140.188.120.255 nmask=255.255.255.0
session setup failed: Call returned zero bytes (EOF)
Any insight into this
2011 Dec 31
1
Histogram omitting/collapsing groups
I have two large datasets (156K and 2.06M records). Each row has the
hour that an event happened, represented by an integer from 0 to 23.
R's histogram is combining some data.
Here's the command I ran to get the histogram:
> histinfo <- hist(crashes$hour, right=FALSE)
Here's histinfo:
> histinfo
$breaks
?[1] ?0 ?1 ?2 ?3 ?4 ?5 ?6 ?7 ?8 ?9 10 11 12 13 14 15 16 17 18 19 20 21
2010 Apr 16
2
managing data and removing lines
Hi,
I am very new to R and I've been trying to work through the R book to gain a
better idea of the code (which is also completely new to me).
Initially I imputed my data from a text file and that seemed to work ok, but
I'm trying to examine linear relationships between gdist and gair, gdist and
gsub, m6dist and m6air, etc.
This didn't work and I think it might have something to do
2008 May 29
1
plotting zoo using datetime as xlim
is there a way to use the actual index value for plotting zoo objects
this is the way that the index is set up and a sample range of what I would
like to plot
01/01/06 00:00:00 - 01/01/06 23:45:00
{
library(zoo)
# chron
library(chron)
fmt.chron <- function(x) {
chron(sub(" .*", "", x), gsub(".* (.*)", "\\1:00", x))
}}
x <- structure(c(15.57, 15.5,
2013 Feb 11
2
FORMAT EDITING
Dear R users,[IF THE FORMAT OF MY EMAIL IS NOT CLEAR, I HAVE ATTACHED A TEXT FILE FOR A CLEAR VIEW]
I would like to use the R output file in Fortran. my file Is exactly in the following format.
ELISA/BOTTO wATER INN
FROM 1900 11 1 TO 1996 12 31
1901.11. 1 447.000
1901.11. 2 445.000
1901.11. 3 445.000
1924.11. 4 445.000
1924.11. 5 449.000
1924.11. 6 442.000
1924.11. 7
2013 Feb 17
6
histogram
HI Elisa,
You could use ?cut()
vec1<-c(33,18,13,47,30,10,6,21,39,25,40,29,14,16,44,1,41,4,15,20,46,32,38,5,31,12,48,27,36,24,34,2,35,11,42,9,8,7,26,22,43,17,19,28,23,3,49,37,50,45)
label1<-unlist(lapply(mapply(c,lapply(seq(0,45,5),function(x) x),lapply(seq(5,50,5),function(x) x),SIMPLIFY=FALSE),function(i) paste(i[1],"<x<=",i[2],sep="")))
2006 Dec 01
3
Make many barplot into one plot
Dear all,
## I have 4 tables like this:
satu <- array(c(5,15,20,68,29,54,84,119), dim=c(2,4),
dimnames=list(c("Negative", "Positive"), c("Black",
"Brown", "Red", "Blond")))
dua <- array(c(50,105,30,8,29,25,84,9), dim=c(2,4),
dimnames=list(c("Negative", "Positive"),
2013 Feb 19
1
data format
Hi,
Try this:
el<- read.csv("el.csv",header=TRUE,sep="\t",stringsAsFactors=FALSE)
?elsplit<- split(el,el$st)
?
datetrial<-data.frame(date1=seq.Date(as.Date("1930.1.1",format="%Y.%m.%d"),as.Date("2010.12.31",format="%Y.%m.%d"),by="day"))
elsplit1<- lapply(elsplit,function(x)
2009 Jan 15
1
Seemingly Unrelated Negative Binomial (SUNB) estimation
Dear all,
I am trying to estimate a system of equations with a Seemingly Unrelated
Regression. However because of the characteristics of the data I'd like to
do it with a negative binomial estimation. Would anybody know how to
implement a Seemingly Unrelated Negative Binomial (SUNB) estimation in R?
Thank you in advance for your help.
Best regards,
Elisa Lanzi
--
Elisa Lanzi
PhD student in