similar to: problem fitting linear mixed models

Dear Ioannis Thank you very much for pointing me to meta-analysis. Although it may not solve my problem with the normalization, it gives me some other options to display the different correlation coefficients. One possibility is the use of Funnel plots, which are even available in library(rmeta). Another possibility is the use of forest-plots, as implemented in rmeta as metaplot. Sorrowly,

Dear all I apologize for cross-posting, but first it is accepted custom to thank the repliers and give a summary, and second I have still the feeling that this problem might be a general statistical problem and not necessarily related to microarrays only, but I might be wrong. First, I want to thank Robert Gentleman, Mark Kimpel and Mark Reiners for their kind replies. Robert Gentleman kindly

I'm working with a nested model (mixed). I have four factors: Patients, Tissue, sex, and tissue_stage. Totally I have 10 patients, for each patient, there are 2 tissues (Cancer vs. Normal). I think Tissue and sex are fixed. Patient is nested in sex,Tissue is nested in patient, and tissue_stage is nested in Tissue. I tried aov and lme as the following, > aov(gene ~ tissue + gender +

Hi, There are 20 subjects grouped by Gender, each subject has 2 tissues (normal vs. cancer). In fact, it is a 2-way anova (factors: Gender and tissue) with tissue nested in subject. I've tried the following: Model 1: lme(response ~ tissue*Gender, random = ~1|subject) Model 2: response ~ tissue*Gender + subject Model 3: response ~ tissue*Gender It seems like Model 1 is the correct one

Hello, I've used this command to analyse changes in brain volume: mod1<-aov(Volume~Sex*Lobe*Tissue+Error(Subject/(Lobe*Tissue)),data.vslt) I'm comparing males/females. For every subject I have 8 volume measurements (4 different brain lobes and 2 different tissues (grey/white matter)). As aov() provides only type I anovas, I would like to use lmer() with type II, however, I have

dear members, i would like to write a variable in a plot title (main="") but i don't know the right syntax:(...i tried a lot of different ways without success. here my example: y=30 z=33 for (i in 10:length(tissue)) { png(filename = tissues[i], width = 1024, height = 768, pointsize = 12, bg = "white") gene.graph("ENSG00000115252", rma.affy, gps=list(1:3,

Hello, I'm using aov() to analyse changes in brain volume between males and females. For every subject (there are 331 in total) I have 8 volume measurements (4 different brain lobes and 2 different tissues (grey/white matter)). The data looks like this: Subject Sex Lobe Tissue Volume subect1 1 F g 262374 subect1 1 F w 173758 subect1 1 O g 67155 subect1 1 O w 30067 subect1 1 P g 117981

Hello, I will start out with the caveat that I'm not a statistician by training, but have a fairly decent understanding of probability and likelihood. Nevertheless, I'm trying to fit a nonlinear model to a dataset which has two main factors using nlme. Within the dataset there are two Type categories and four Tissue categories, thus giving me 8 datasets in total. The dataset is in

Dear all, I would like to know if it is possible to fit in R a Cox ph model with time-dependent covariates and to account for hierarchical effects at the same time. Additionally, I'd like also to know if it would be possible to perform any feature selection on this model fit. I have a data set that is composed by multiple marker measurements (and hundreds of covariates) at different time

Hi, Thanks in advance for any advice you can give me, I am very stumped on this problem... I use R every day and consider myself a confident user, but this seems to be an elementary problem.. Outline of problem: I am analysing the results of a study on protein expression in cancer tissues. I have raw intensities from 2 different types of cancer and normal tissue, which can be taken from several

Hi, I've lost my mind on it... I have to scatterplot two vectors, grouped by a third variable, with two different dimensions according to whether each cell line in the plot is sensitive or resistant to a given drug, and with a different color for each of 9 tissues of origin. Here's what I've done:

I cannot find in the literature a way to conduct the following t.test on 2 objects, A and B A B col1 col2 col3 col1 col2 col3 Where col(i)'s name is identical in both A and B (they are names of tissues). How do I test (t.test) if each tissue across the object is signifanctly different?? (i'm pretty sure I have to use tapply())

Dear R-users I am relatively new to R, i hope my many novice questions are welcome. I have problems accessing some objects (specifically the random effects, correlation structure and variance function) from an object of class gls and lme. I used the following models: yah <- gls (outcome~ -1 + as.factor(Trial):as.factor(endpoint)+

Hello I'm fitting a gls model with a variance-covariance structure and an getting an error message I don't understand I'm using gls() from the nlme library with the structure defined by correlation = corSymm(form = ~1|Subject), weights = varIdent(form=~1|strata) I get the error Error in recalc.corSymm(object[[i]], conLin) : NA/NaN/Inf in foreign function call (arg 1) My

I was reading the help but just did not get how to specify starting values for varIdent() of the lme() function, although I managed to do it for corSymm(). Do I specify the values just as they are printed out in an output, like c(1, 1.3473, 1.0195). Or do I need to take the residual and multiply it with these like c(0.2235, 0.2235*1.3473, 0.2235*1.0195) or any other form that I dont know of?

Hello, I have used nlme to fit a model, the R syntax is like fmla0<-as.formula(paste("~",paste(colnames(ldata[,9:13]),collapse="+"),"-1")) > fmla1<-as.formula(paste("~",paste(colnames(ldata[,14:18]),collapse="+"),"-1")) >

Dear R-Users I have a problem accessing some values in the output from the summary of an lme fit. I fit the model below: ggg <- lme (ST~ -1 + as.factor(endp):Z.sas + as.factor(endp), data=dat4a, random=~-1 + as.factor(endp) + as.factor(endp):Z.sas|as.factor(trials), correlation = corSymm(form=~1|as.factor(trials)/as.factor(id)), weights=varIdent(form=~1|endp)) hh

Hello, I have a simulated data structure in which students are nested within teachers, and with each student are associated two test scores. There are 20 classrooms and 25 students per classroom, for a total of 500 students and two scores per student. Here are the first 10 lines of my dataframe "d": studid tchid Y time 1 1 1 -1.0833222 0 2 1 1

Hi, I am doing an analysis to see if these is tissue specific effects on the gene expression data . Our data were collected from 6 different labs (batch effects). lab 1 has tissue type 1 and tissue type 2, lab 2 has tissue 3, 4,5,6. The other labs has one tissue type each. The 'sample' data is as below:

Hello and thanks in advance I am running a glm in R the code is as follows with residual diagnostic code below model4<-glm(Biomass~(Treatment+Time+Site)^2, data=bobB, family=quasi(link="log", variance="mu")) par(mfrow=c(2,2)) plot(model2) to test the effect of grazing exclusion of feral horses for a Phd with following factors: Treatment - 3 levels which are grazed