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...I want each comparison to only be listed once, the difference between the 1st year the sample was taken and then 2nd year the sample was taken. (This would probably entail just taking a portion of the matrix.) . This is what the result would be difftype value x1x2 x1x3 x1x4 x1x5 x2x3 x2x4 x2x5 x3x4 x3x5 x4x5 Thank you for any help you can give me. -- View this message in context: http://r.789695.n4.nabble.com/how-to-create-a-substraction-matrix-subtract-a-row-of-every-column-from-the-same-row-in-other-column-tp4642949.html Sent from the R help mailing list archive at Nabble.com.