search for: antimicrobials

Hi, I've been doing an experiment, measuring the dead-zone-diameters of bacteria, when they've been grown with paper diffusion disks of antimicrobial. There are two groups, or treatments - one is bacteria that have been cultured in said antimicrobial for the past year, the other group is of the same species, but lab stock and has not gone had any prior contact with the antimicrobial.

...the multiple readings from one disk if eel, is pseudoreplication. I've also read about including Error() terms in a formula. I'm unsure of the two NE. Treatments comming from the same culture does not introduce pseudoreplications at Treatment Factor Level, because of the two different antimicrobials used have two different effects. I was hoping for a more expert opinion on whether I have identified pseudoreplication correctly or if there is indeed pseudoreplication in the 5 Lineages or anywhere else I haven't seen. And how best this is dealt with in R. At the minute my solution to the...

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...pseudoreplication. I've also read about including Error() terms >> in a formula. >> >> I'm unsure of the two NE. Treatments comming from the same culture does not >> introduce pseudoreplications at Treatment Factor Level, because of the two >> different antimicrobials used have two different effects. >> >> I was hoping for a more expert opinion on whether I have identified >> pseudoreplication correctly or if there is indeed pseudoreplication in the 5 >> Lineages or anywhere else I haven't seen. And how best this is dealt with i...

Respected Sir/Madam, I have a question regarding calculation of LD50 (Lethal Dose) and IC50 (50% inhibitory concentration) of an antimicrobial experiment. I have used a compound isolated from a plant and observed its effect on the fungus *Fusarium oxysporum* by the food poisoning method. Solutions of the compound at concentrations of 0, 50, 100, 150, 200 and 250µg/ ml were added to

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Hi, I'm doing some modelling (lm) for my 3rd year dissertation and I want to do some resampling, especially as I'm working with microbes, getting them to evolve resistance to antimicrobial compounds, and after each exposure I'm measuring the minimum concentration required to kill them (which I'm expecting to rise over time, or exposures), I have 5 lineages per cleaner, and

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-------- Original Message -------- Subject: Re: [R] Simulation - Natrual Selection Date: Wed, 05 Jan 2011 17:24:05 +0000 From: Ben Ward <benjamin.ward@bathspa.org> To: Bert Gunter <gunter.berton@gene.com> CC: Mike Marchywka <marchywka@hotmail.com> On 05/01/2011 17:08, Bert Gunter wrote: > Couple of brief comments inline below. -- Bert > > On Wed, Jan 5, 2011 at

Hi, I wonder if anyone could advise me with this: I've been trying to make a standard curve in R with lm() of some standards from a spectrophotometer, so as I can express the curve as a formula, and so obtain values from my treated samples by plugging in readings into the formula, instead of trying to judge things by eye, with a curve drawn by hand. It is a curve and so I used the