Dear R-users, I have the following problem. I have performed an experiment for which I gathered a lot of data which I now want to test. The problem is that I cannot find an appropriate test in R (I am a starter) and someone might give me a hand. This is what I have done: Across three sites (Site), I have laid out five transects (Trans)...meaning five transects in each sites. In each transect I have five Microhabitats (MH) which should be regarded as subplots (I think). In each transect, every MH has the same position (so they are not randomized). I now want to test the effect of Site and MH (nested in Trans) on my response variables. This is what I do now: model<-aov(Response~Site*MH+error(Trans/MH)) I get the following output: Error: Trans Df Sum Sq Mean Sq Site 1 0.030294 0.030294 Error: Trans:MH Df Sum Sq Mean Sq Site 1 10.8367 10.8367 MH 3 0.2836 0.0945 Error: Within Df Sum Sq Mean Sq F value Pr(>F) Site 2 0.92504 0.46252 11.7304 5.880e-05 *** MH 4 1.86688 0.46672 11.8370 5.645e-07 *** Site:MH 8 1.17041 0.14630 3.7105 0.001615 ** Residuals 54 2.12917 0.03943 --- Signif. codes: 0 ?***? 0.001 ?**? 0.01 ?*? 0.05 ?.? 0.1 ? ? 1 How do I read this? Any help appreciated! BTW: I also tried the lme function: model<-lme(Response~Site*MH, random=~1|Trans/MH) but then the output is really complicated. ----- Dr. Bjorn JM Robroek Ecology and Biodiversity Group Institute of Environmental Biology, Utrecht University Padualaan 8, 3584 CH Utrecht, The Netherlands Email address: b.j.m.robroek at uu.nl http://www.researcherid.com/rid/C-4379-2008 Tel: +31-(0)30-253 6091 -- View this message in context: http://r.789695.n4.nabble.com/Nested-design-tp3557388p3557388.html Sent from the R devel mailing list archive at Nabble.com.