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Hi My name is Rocky and I am trying to use the org.Dm.eg.db library. When I am using the org.Dm.egFLYBASE2EG[fb_ids] it is stopping at a point where it cannot find any value for a given ID such as the following: Error in .checkKeys(value, Rkeys(x), x@ifnotfound) : value for "FBgn0004461" not found Then the whole thing stops. I cannot retrieve any information on the values that has been

I am attempting to created colClasses for several tables, then read only specific columns. There are two different table layouts that I am working with. If I use exclusively one layout, the script works perfectly, but if I mix the layouts, it fails to grab the correct columns form layout that is read in second. It appears that colClasses fails to adopt the new structure after the first iteration.

Dear Sirs, How can I revert to an older limma version? Typing "install.packages("limma")" in R gives a list of mirrors. How can I install the version I want after I obtain and untar the file (e.g, limma_2.9.1.tar.gz)? I am running R 2.5.0 on a Linux machine (CentOS 5). When using limma it will not go past the read.maimages command. I get this error: Error in

Hi, there. I used the function read.maimages in limma package to analyze the microarray data .but I got following message >RG <- read.maimages(targets$FileName, source="spot") Error in grep(pattern, x, ignore.case, extended, value, fixed) : invalid argument I don't know what is the matter Thanks a lot Regards Shizhu Zang Department of Biochemsitry Peking

Dear mailing group, This is my first time here. Glad to have this resource! I am currently trying to load an Excel file into R (limma package loaded) using the source(*name of directory*) command, but it cannot open the file. I renamed the file as .R and .RData, to no avail. The Excel data contains one gene name per row and about 100 data points per gene (columns). I am only used to

The inst/doc directory of the DAAG package has 6 files coral551.spot, ... that are around 0.85 MB each. It would be useful to be able to zip then, but that as matters stand interferes with the use of the Sweave file that uses them to demonstrate input of expression array data that is in the "spot" format. They do not automatically get unzipped when required. I have checked that

Hi, I am following the protocol outlined here for analysis of single channel Agilent microarray data: http://matticklab.com/index.php?title=Single_channel_analysis_of_Agilent_microarray_data_with_Limma I keep getting the following error message when using Limma's read.maimages function to load my data into an RGList object: Error in RG[[a]][, i] <- obj[, columns[[a]]] : number of

Hi Everyone, I have been trying to read some Arrayvision files( 2 channel cDNA) and am having some problem. My code is : setwd('C:/work/data/limma/ndd1'); files <- c('ndd1_1.txt','ndd1_2.txt','ndd1_3.txt'); RG=read.maimages(files,"arrayvision",sep="\t"); #Normalisation MA=normalizeWithinArrays(RG); #plotPrintTipLoess(MA); #Fit Linear

Hi! Anyone can help how I can do LVS normalization starting from an EList.raw created using: data= read.maimages(files, green.only=T, columns= list(E='gMedianSignal',Eb='gBGUsed')) thanks in advance

Hi, I'm working with custom slides(Cy5) and working in the normalization of the arrays. I have three arrays (technical replicates). I have sucesfully normalized the data using vsn, however i would like to normalize using spike in controls. My controls are annotated as CTL-1 to x and i would like to do etiher a normalization by block per array or the mean of all the controls per array. The gal

Dear list members, I am analysing my microarray data using limma package. Now I encounter several problems. Looking forward to your suggestions! Question 1: During the process of background correction using method="normexp", four warning messages appeared as "NaNs produced in: log(x)" (as you can see in the program posted below). What does that mean? How will it effect the