similar to: Need to build package for Affy HT HG-U133+ PM arrays

Hi, I am a Ph.D. student from Québec, Canada. I’m a beginner with R and Bioconductor. Until now the only experience I have is in analyzing microarray data using affy and limma packages. Now I am trying to analyze Rat Gene 10 st arrays and I would like to run RMA analysis and Smyth moderated t test on those arrays. Since no cdf official package is available for those arrays, after reading many

Dear maintainer, I used bioconductor package, pdInfoBuilder, to make a microarray platform annotation package. I named this package, pd.pdinfo.gpl11164.ndf.txt. This "self-made package" is to be used with oligo package. Prior to using oligo package, I need to install the annotation package, pd.pdinfo.gpl11164.ndf.txt, and cannot just copy it to the library tree. I tried using the

Hi everyone I have the following problem: My dataframe has 3 variables: ID, Year and and an outcome variable. The dataframe contains repeated measurements because the subjects filled out a questionnaire every year. The time span covers 2 years. Now I want to check if there is a significant change in the outcome over the 2 years with a paired wilcox.test. The problem: Not every subject

When I try to load cel files (hgu133a) using the ReadAffy() in R 1.8.1 command I get an error message: > x<-ReadAffy() Error: cannot allocate vector of size 102973 Kb Does anybody know what does this error mean and how to overcome it? I have tried to load the same data with R 1.7.1 and it works. There is also no error when I use R 1.8.1 to load moe430 cel files. Thanks very much for any

Hi, i was looking into the documentation for the rma() function in affy() package, and was trying to figure out how exactly the background normalization is done. I read all three papers cited in the rma() documentation, but the most detailed explanation i could find was in Irizary et al., 2003, where they state that they compute B(PM_{ijn}) = E[s_{ijn} | PM_{ijn}] where s_{ijn} is assumed to

Dear all, I was trying to normalize a subset of affy data those transcribe are either P or M (called pm_filter). I am able to normalize pm_filter subset by using RMA method, however MAS5 is not working. For RMA method, I used the following commend: est<-rma(affydata, subset=pm_filter). Could any help me; how do I do this by using MAS5 method? Regards, Anup Som -- Dr. Anup Som,

Dear All, There is a question I met when using Affy package in Bioconductor. I asked it in BioC and didn't get any responses. Sorry to post again: Could anyone tell me how to draw a deep-blue Affymetrix image through Image() function in Affy package? The default settings of image() draw me a black-white image and if I modify it to 256 colors, I get a somehow yellowish image. The reason

Hi , I was trying read in an affy array/matrix and then to calculate the mean value for all the duplicates. Is there a function in the R package that would do this? I tried the help function and searched for 'duplicate'. Although it provides a list of functions that would eliminate the duplicate probe ids, I couldn't find one that would calculate the mean for the duplicates. Any

Does anyone have nice quality controlls for affy arrays,.... Can't find any tools as are being used for 2 dye arrays..... cheers, marinus This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html [[alternative HTML version deleted]]

Hi! I would like to select probes (affy expression set) of genes that are "cancer-related". Conventionally the decision whether a gene is cancer-related or not is made by looking up the literature. Since this is not possible for all genes on the array I wonder if there is a way of doing this automatically? Best wishes Kristian -- _____ Dr Kristian Unger Imperial College London

Hi all, I tried to do normalization of affymetrix data with bioconductor on a Linux server. When I read in the cel files all seemed ok. But the next step caused an error. With Win XP all works fine. Did anyone experience similar problems? Thanks, Thomas > PI <- ReadAffy() > PI AffyBatch object size of arrays=712x712 features (14 kb) cdf=ATH1-121501 (??? affyids) number of

Hello, I have a problem with read.affy.mixed function. I want to read in together a set of CEL files from chip types Affymettrix HGU133A_2 and HGU133_Plus_2. I have my files to be read in in one directory together with a white space delimited file describing them (covdesc). In this directory I give a command: > merge <- read.affy.mixed() Error in merged[[i]] : subscript out of

Hello All, I need your help. I am analysing affymetrix data and have to select the probe-set that has median expression among all the probe-sets for same gene. This way I want to remove the redundancy by keeping the analysis to single gene entry level. I am fully aware that it is not a nice thing to do but I just have to do it. To do so, I came across 'findLargest' function of

Hi, I have been using the prcomp function to perform PCA on my example microarray data, (stored in metric text files) which looks like this: 1a 1b 1c 1d 1e 1f ...................................................4r 4s 4t g1 1.2705 1.2766 ...........................................................2.0298 g2 0.1631

Hello, I am trying to install affy package in R as follow: >R CMD INSTALL -l lib ~/rstuffs/affy_1.4.32.tar.gz Then I get an error at the end: Warning message: There is no package called 'Biobase' in: library(package, character.only = TRUE, logical = TRUE, warn.conflicts = warn.conflicts, [1] "ProgressBarText" [1]

Hi, I have a grandstream HT 503 ATA which seems to support TLS/SRTP. I generated the keys and certificates for both the server and the device as mentioned at https://wiki.asterisk.org/wiki/display/AST/Secure+Calling+Tutorial I then copied the grandstream.crt and grandstream.key over to the ATA. Unfortunately, the device is refusing to register and in asterisk cli i see SSL certificate ok ==

Hello there, I just wasted some time setting up a Grandstream HT-488 to be used with Asterisk, so I thought I'd share the experience by writing a small tutorial at: http://astrecipes.net/index.php?n=338 Most tutorials I came across were for old versions of the firmware, and I spent too much time trying to figure out what the Forward to VoIP option wanted.... :-( I hope this helps,

Just to tease people I'll be post a URL to my review of the HT-286 by thursday..

Hi List, Just received a HT-286 Analog Telephone Adapter. This device is allows the user to take a standard analog telephone set and connect it to a VoIP/SIP based gateway. A low cost way of having PSTN devices make use of VoIP/SIP services. For example you could take a credit card processing machine and punch it into a location where you have broad band access. You could take a FAX

Hi, Can I use HT-486 as VoIP Gateway together with Asterisk? Are there any success experiences? -- Best Regards, Miroslav Nachev