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My script: raw = read.table("c:\\Documents and Settings\\protein\\My Documents\\My Fragments\\file1.txt", header = TRUE) #Normal Average Signal VS Pool jpeg("Normal Average Signal vs Pool_test.jpg", width = 800, height = 600) xx_norm = raw[,3] yy_norm = rowMeans(raw[,4:13]) plot(xx_norm, yy_norm) dev.off() I confirmed that the data base works and the plot function does work on its own. But whenever I try running it and output to jpg, it gives me "null device" the whole script was working just earlier. thanks for the help. -- Edward...