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...ch comparison to only be listed once, the difference between the 1st year the sample was taken and then 2nd year the sample was taken. (This would probably entail just taking a portion of the matrix.) . This is what the result would be difftype value x1x2 x1x3 x1x4 x1x5 x2x3 x2x4 x2x5 x3x4 x3x5 x4x5 Thank you for any help you can give me. -- View this message in context: http://r.789695.n4.nabble.com/how-to-create-a-substraction-matrix-subtract-a-row-of-every-column-from-the-same-row-in-other-column-tp4642949.html Sent from the R help mailing list archive at Nabble.com.