Displaying 1 result from an estimated 1 matches for "vsnmatrix".
Did you mean:
dsymatrix
2009 Mar 13
0
Singal channel spike in controls with custom microRNA slides - Normalization help needed
...ot;genepix",columns=list(R=Cy5,G=Cy5, Rb=Cy5b,
Gb=Cy5b))
RG$G <- NULL
RG$Gb <- NULL
RG$genes <- readGAL("array_human_mirs.gal")
#Here are my spike in controls for normalization
isSpikeIn <- grep("CTL", RG$genes$Name)
#The vsn normalization works fine
mat <- vsnMatrix(RG$R)
However i would like to normaliza using my spikein controls by block or by
using the mean of all controls.
Could you help on that ??
thanks,
david
[[alternative HTML version deleted]]