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...iment, I selected plots of land within a forest either with honeysuckle or without honeysuckle. Thus, my main factor is fixed, with 2 levels: "honeysuckle present"(n=11) and "honeysuckle absent"(n=8). Within each plot of land, I have a "trenched" subplot and an "untrenched" subplot. Within each subplot of every plot, I measured soil moisture. Now I need to do a nested Anova to compare the soil moisture values between treatments. I don't really want to discard some data to make the sample sizes balanced. Does anyone know how to do a nested, unbalanced An...