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2004 Nov 12
0
Design Matrix
...need some help on matrix design and B statistics by using limma package.
I want to compare gene expression in 2 groups of cDNA samples.
The experiment compares 4 treated mice(#1,#2,#3,#4) and 4 control mice
(#5,#6,#7,#8).
The target file is
FileName Cy3 Cy5
mice1.spot Control_#5 Treat_#1
mice2.spot Treat_#1 Control_#5
mice3.spot Control_#6 Treat_#2
mice4.spot Treat_#3 Control_#7
mice5.spot Control_#8 Treat_#4
The first slide (mice1.spot) and the second slide(mice2.spot) are
dye-swap. There is no common reference. There are 3 replicated spots of
each gene on e...