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...n I try to rbind the output from strsplit. please let me know if I'm missing something obvious, thanks, alison here are my commands: >strsplit<-strsplit(as.character(Rumino_Reps_agreeWalign$geneid),"\\.") > Rumino_Reps_agreeWalignTR<-transform(Rumino_Reps_agreeWalign,taxid=do.call(rbind, strsplit)) Warning message: In function (..., deparse.level = 1) : number of columns of result is not a multiple of vector length (arg 1) here is my data: > head(Rumino_Reps_agreeWalign) geneid count_Conser count_NonCons count_ConsSubst 1 657313.loc...