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...629 0.137 ... $ first : num 0.281 0.539 0.668 0.438 0.137 ... $ second: num 0 1.246 0.562 0.191 0 ... # Now variable "Cond" still has 4 levels but with which I have excluded one level! #Whether I apply at this point interaction plot, the graph considers 4 levels of which. attach(sentence_trial) x11() interaction.plot(Cond,fam,total) # Where is the problem? Thanks in advance. Nicola