search for: rnaseq

Displaying 3 results from an estimated 3 matches for "rnaseq".

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2017 Jun 09
2
Dendogram from RNAseq read count to show correlation between biological replicate using R
Dear all, I need to make dendogram from read count in a csv file across 34 samples including biological replicate. Please share R code or package to do this. Do I also need to normalized read count before using read data? Thanks [[alternative HTML version deleted]]
2011 Nov 07
1
DESeq
Hello, I have RNAseq data, which I am trying to analyze with DESeq. My file (tab delimited .txt) appears to be correct: >head(myfile) VZ_w13 VZ_w14a VZ_w14b VZ_w15a VZ_w15b VZ_w16a ENSG00000253101 0 0 0 0 0 0 ENSG00000223972 0 0 0 0 0 0......
2012 Nov 17
1
fold change calculation
Hi, I am really new to edge R and I have used it to calculate gene expression with RNASeq data comparing 2 different conditions. I used a P value of 0.05 and I got a list of DGE contigs up and down regulated. WhatI was wondering is how to convert the logFC value that appear in the output of the exact test. Is it a log2 base? Is fold 2 change considered as cutoff? I am trying to find thi...