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...g the protocol outlined here for analysis of single channel Agilent microarray data: http://matticklab.com/index.php?title=Single_channel_analysis_of_Agilent_microarray_data_with_Limma I keep getting the following error message when using Limma's read.maimages function to load my data into an RGList object: Error in RG[[a]][, i] <- obj[, columns[[a]]] : number of items to replace is not a multiple of replacement length I think this may be due to my Agilent raw data txt files being in the wrong format. I am having difficulty finding an example Agilent feature extraction raw data txt fil...

....txt Error in switch(method, loess = { : Layout argument not specified I then created a layout file and added in the following code, but I still got the same error message. RG$genes=readGAL(); gal=readGAL(); layout=getLayout(gal); Also, when I type RG , I get, > RG An object of class "RGList" $R [1] 3 $Rb [1] 6 $G [1] 10 $Gb [1] 14 $targets FileName ndd1_1 ndd1_1.txt ndd1_2 ndd1_2.txt ndd1_3 ndd1_3.txt $source [1] "arrayvision" $genes Block Row Column ID Name 1 1 1 1 my_ID my_Description 2 1 1 2 my_ID my_Description 3...