search for: pk3

...concentration I can calculate accurately the pH if I know 3 pK values for HEPES by finding the single root with uniroot Now, the problem is that there is some disagreement in the literature what is the correct value for the 3 pKs. I know the 3 pK values have the relationship pK1 < pK2 <- pK3 and for the most common formulation of HEPES I know the charge on fully protonated species is 2. Hence I can generate a huge number of pK values from uniform distribution by taking sort(runif(3,-1,9) and make sure there are no ties and then run all those triplets of pK values to find pH values...

...which delivers charge in the > fluid for known pKs, HEPTOT and SID. Is it possible to have > root-finding in the formula with nls? Sure. Just reformulate the problem in terms of a function that takes a vector of predictors (your independent variable SID) and the desired parameters (pK1, pK2, pK3) as separate arguments and then returns predicted values of the dependent variable (to compare against pHobs): kw <- 1e-14 # I'm assuming pHm <- Vectorize(function(SID, pK1, pK2, pK3) -log10(uniroot(HEPESFUNC,c(1e-20,1),tol=1e-20,maxiter=1e4, HEPTOT=HEPTOT,SID = SID, pK1=pK1,pK2...

Thanks a lot! This was amazing. I'm not sure I see how the conditiion pK1 < pK2 < pK3 is enforced? - it comes from the derivation via generalized Henderson-Hasselbalch but perhaps it is not really necessary. Anyway, the use of Vectorize did the trick! Best wishes Troels Den 06-11-2023 kl. 19:19 skrev Ivan Krylov: > ? Mon, 6 Nov 2023 17:53:49 +0100 > Troels Ring <tring a...

...d that is well up to the job (using nvidia acceleration etc....v177) There are some funny reports in the below message... Can anyone help? Regards Si COD 1.3 build win-x86 Mar 2 2004 ----- FS_Startup ----- Current language: english Current search path: C:\Program Files\Call of Duty\main\pakb.pk3 (60 files) C:\Program Files\Call of Duty\main\paka.pk3 (41 files) C:\Program Files\Call of Duty\main\pak9.pk3 (149 files) C:\Program Files\Call of Duty\main\pak8.pk3 (235 files) C:\Program Files\Call of Duty\main\pak6.pk3 (3 files) C:\Program Files\Call of Duty\main\pak5.pk3 (4858 files) C:\Program...

...om below. Note that nls() can only handle bounds in the "port" algorithm, and the man page rather pours cold water on using that algorithm. Best, JN On 2023-11-06 14:43, Troels Ring wrote: > Thanks a lot! This was amazing. I'm not sure I see how the conditiion pK1 < pK2 < pK3 is enforced? - it comes from the > derivation via generalized Henderson-Hasselbalch but perhaps it is not really necessary. Anyway, the use of Vectorize > did the trick! > > Best wishes > Troels > > Den 06-11-2023 kl. 19:19 skrev Ivan Krylov: >> ? Mon, 6 Nov 2023 17:5...

G'day Troels, On Mon, 6 Nov 2023 20:43:10 +0100 Troels Ring <tring at gvdnet.dk> wrote: > Thanks a lot! This was amazing. I'm not sure I see how the conditiion > pK1 < pK2 < pK3 is enforced? One way of enforcing such constraints (well, in finite computer arithemtic only "<=" can be enforced) is to rewrite the parameters as: pK1 = exp(theta1) ## only if pK1 > 0 pK2 = pK1 + exp(theta2) pK3 = pk2 + exp(theta3) And then use your optimiser to optimise...

...guys, plz help... i am trying to play American McGee's Alice(tm) (its from 2000), and i installed it. but when i try to run it i get: Code: American McGee's Alice win-x86 Nov 13 2000 ----- FS_Startup ----- Current search path: Z:\media\Windows\Games\Ububntu\Alice (2000)\base\pak4_english.pk3 (44 files) Z:\media\Windows\Games\Ububntu\Alice (2000)\base\pak3.pk3 (9 files) Z:\media\Windows\Games\Ububntu\Alice (2000)\base\pak2.pk3 (71 files) Z:\media\Windows\Games\Ububntu\Alice (2000)\base\pak1_large.pk3 (3368 files) Z:\media\Windows\Games\Ububntu\Alice (2000)\base\pak0.pk3 (7723 files) Z:\...

Thanks a lot, Berwin. Unfortunately, pK1 may well be negative and as I understand the literature it may be poorly defined as such, and also seems to be at a boundary, since when lower is set to say rep(-4,3) pK1 is returned as -4 while pK2 and pK3 are undisturbed. Perhaps the point is that pK1 is not carrying any information at the pH around 5. Fair enough, I guess. Only, I believe I need stick to including all three pK values to be in agreement with the molecular information about HEPES - although even this is contentious. Be as it may,...

...er. The FAQ says it's not possible to install drivers in WINE, so... I'm stuck. Any help available? Here's the full log from the Wolf Console: Code: Wolf 1.32 win-x86 May 1 2002 ----- FS_Startup ----- Current search path: C:\Program Files\Return to Castle Wolfenstein\main\sp_pak3.pk3 (14 files) C:\Program Files\Return to Castle Wolfenstein\main\sp_pak2.pk3 (232 files) C:\Program Files\Return to Castle Wolfenstein\main\sp_pak1.pk3 (1342 files) C:\Program Files\Return to Castle Wolfenstein\main\pak0.pk3 (4775 files) C:\Program Files\Return to Castle Wolfenstein/main ------------...

...-c(-0.2061826,0.5888406,0.2026079,1.0000000,0.2342754,0.6865078,-0.1265754) x <- c(1,2,3,4,5,6,7) p <- nls(m~k1*x+k2*cos(x)+k3*sin(x)+k4*cos(2*x)+k5*sin(2*x)+k6*cos(3*x),start = list(k1=0,k2=0,k3=0.1,k4=0.1,k5=0,k6=0)) par ?<- c(pk1=summary(p)$parameters[1,1],pk2=summary(p)$parameters[2,1],pk3=summary(p)$parameters[3,1],pk4=summary(p)$parameters[4,1],pk5=summary(p)$parameters[5,1],pk6=summary(p)$parameters[6,1]) xx <- seq(1,7,length.out=500) mm <- par[1]*xx+par[2]*cos(xx)+par[3]*sin(xx)+par[4]*cos(2*xx)+par[5]*sin(2*xx)+par[6]*cos(3*xx) plot(x,m) points(xx,mm,type="l") ?...

...n = data[(1+4*(i-1)):(4*i)] p = theta[(1+3*(i-1)):(3*i)] P = 1-sum(p) S[(1+3*(i-1)):(3*i)] = n[1:3]/p-n[4]/P } S } #where theta=(p11,p12,p13,p21,p22,p23,...,pK1,pK2,pK3). #The function Rmat calculates the restriction matrix needed for constrained estimation Rmat = function(k) { R = matrix(1,4,3) R[1,2] = R[1,3] = R[2,3] = R[3,2] = 0 RR = cbind(-R,R) RRR = matrix(0,4*(k-1),3*k) for (...

...ting 768 bit RSA key. RSA key generation complete. debug1: Server will not fork when running in debugging mode. get_sock_port: getnameinfo NI_NUMERICSERV failed debug1: Calling cleanup 0x12005c9c0(0x0) Port 22 is open and there is not another copy of sshd running. 3.5p1 works fine Os: Tru64 5.1a pk3 Platform: GS160, Alpha EV6.8 Generic install No options were passed to the configure process. Please let me know if I can be of anymore help. Darin Fisher darin_fisher at hotmail.com ------- You are receiving this mail because: ------- You are the assignee for the bug, or are watching the a...

Hello list. I'm trying to rebuild the 2.6.9.67.0.20.EL kernel, but it fails even without modifications. How did I try it? Created a (non-root) build environment (not a mock ) Installed the kernel.scr.rpm and did a rpmbuild -ba --target=`uname -m` kernel-2.6.spec 2> prep-err.log | tee prep-out.log The build failed at the end: Processing files: kernel-xenU-devel-2.6.9-67.0.20.EL Checking