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p11t
2007 Aug 14
0
Limma - 2x2 factorial design matrix
...example of design matrix, Mt for Weaver data set in LimmaGUI help file. Is the design matrix looking at the effect of different time point for mutant sample? Mt11 and Mt21 refer to the effect of mutant at day 11 and day 21 alone? What is the logically theory behind to asign 1 or -1 to slides P21WT.P11WT for effect of Mt11 and Mt21? Does it has anything to do with the vector? Or we will subtract it with Wt at both time point in order for us to look at the effect of Mt11 and Mt21?
I use Lucidea Universal ScoreCard as my spike in control. Do I need to flag them out before I carry out the analysis?...