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...a rather than all 9. > strain.data <- read.xlsx("Dee rhiz.xlsx", sheetName="strain", header = T, row.names = 1) > env.data <- read.xlsx("Dee rhiz.xlsx", sheetName="env", header = T, row.names = 1) > strain.cca <- cca(strain.data ~ Ph+TotalN+Organicmatter+Ca+K+Na+P+Cu+Mn, data=env.data) > strain.cca Call: cca(formula = strain.data ~ Ph + TotalN + Organicmatter + Ca + K + Na + P + Cu + Mn, data = env.data) Inertia Proportion Rank Total 5 1 Constrained 5 1 5 Unconstrained 0...

...row.names = 1) str(strain.data) # lists 9 columns at the end with all NAs strain.data1 <- (strain.data, sqrt.dist = TRUE) # this is not a valid R line. I get Error: unexpected ',' in "strain.data1 <- (strain.data," strain.cca1 <- cca(log1p(strain.data) ~ Ph + TotalN + Organicmatter + Ca + K + Na + P + Cu + Mn, env.data) # More errors resulting from all the NA values Error in Math.data.frame(strain.data) : non-numeric variable in data frame: NA..1NA..2NA..3NA..4NA..5NA..6NA..7NA..8NA..9 You have only 6 observations. In strain.data, since each column has a single 1 and t...