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...+1 } } return(y) } but this code actually is not working, it reads only one sequence, i dont know why the loop is not working for the "i", which suppose to read the nine rows of the file sequence.txt. the sequence.txt file is attached to this message. cheers http://n4.nabble.com/file/n997072/sequence.txt sequence.txt -- View this message in context: http://n4.nabble.com/caculate-the-frequencies-of-the-Amino-Acids-tp997072p997072.html Sent from the R help mailing list archive at Nabble.com.