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2011 Jan 19
1
A question regarding volcano plot
...eal data, using log2(ratio) vs. Z-value significance. However the scatter of the points is too less contrary to 'normal' volcano plots and I'm getting a sharp 'V' shaped plot. *Am I doing something wrong here?* The data(ratio) is available from pastebin: http://pastebin.com/m2Jss3qF data <- read.table("data.txt",header=FALSE) ratio <- data$V1 ratio.mean <- mean(ratio) ratio.sd <- sd(ratio) ratio.log <- log2(ratio) z <- (ratio-ratio.mean)/(ratio.sd) z.sig <- 2*pnorm(-abs(z)) z.tsig <- 2*pt(-abs(z),df=length(ratio)-1)#sig from t-dist par(mfr...